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Literature summary for 3.1.2.16 extracted from

  • Giffhorn, F.; Gottschalk, G.
    Inactivation of citrate lyase from Rhodopseudomonas gelatinosa by a specific deacetylase and inhibition of this inactivation by L-(+)-glutamate (1975), J. Bacteriol., 124, 1052-1061.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
citrate induced by Rubrivivax gelatinosus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.01 M, 40% inhibition Rubrivivax gelatinosus
L-arginine 0.01 M, 47% inhibition Rubrivivax gelatinosus
L-aspartate
-
Rubrivivax gelatinosus
L-glutamate
-
Rubrivivax gelatinosus
additional information D-glutamate, 2-oxoglutarate, L-alpha-hydroxyglutarate and L-proline are less effective inhibitors, iodoacetate, iodoacetamide or N-ethylmaleimide does not interfere with deacetylase activity Rubrivivax gelatinosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
[citrate (pro-3S)-lyase](acetyl form) pH 7.2, 30°C Rubrivivax gelatinosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[citrate (pro-3S)-lyase](acetyl form) + H2O Rubrivivax gelatinosus
-
[citrate (pro-3S)-lyase](thiol form) + acetate
-
?

Organism

Organism UniProt Comment Textmining
no activity in Enterobacter aerogenes
-
-
-
no activity in Streptococcus diacetilactis
-
-
-
Rubrivivax gelatinosus
-
DSM 149
-

Purification (Commentary)

Purification (Comment) Organism
-
Rubrivivax gelatinosus

Storage Stability

Storage Stability Organism
-15°C, purified enzyme stable for at least 6 months Rubrivivax gelatinosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acetyl-CoA, acetoacetyl-CoA, S-acetyl-N-acetylcyseamine and purified citrate lyases from Enterobacter aerogenes and Streptococcus diacetilactis are no substrates, reaction with butyryl-S-citrate lyase, propionyl-S-citrate lyaseand acetyl-S-citrate lyase only slightly faster than spontaneous hydrolysis Rubrivivax gelatinosus ?
-
?
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
Rubrivivax gelatinosus [citrate (pro-3S)-lyase](thiol form) + acetate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
-
Rubrivivax gelatinosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2 8.1 because of the instability of the substrate acetyl-S-citrate lyase at acidic and alkaline pH-values, all assays are carried out at pH 7.2, where the spontanous hydrolysis of the substrate is negligible and the enzyme shows 70% of the activity at pH 8.1 Rubrivivax gelatinosus