Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | induced by | Rubrivivax gelatinosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.01 M, 40% inhibition | Rubrivivax gelatinosus | |
L-arginine | 0.01 M, 47% inhibition | Rubrivivax gelatinosus | |
L-aspartate | - |
Rubrivivax gelatinosus | |
L-glutamate | - |
Rubrivivax gelatinosus | |
additional information | D-glutamate, 2-oxoglutarate, L-alpha-hydroxyglutarate and L-proline are less effective inhibitors, iodoacetate, iodoacetamide or N-ethylmaleimide does not interfere with deacetylase activity | Rubrivivax gelatinosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
[citrate (pro-3S)-lyase](acetyl form) | pH 7.2, 30°C | Rubrivivax gelatinosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[citrate (pro-3S)-lyase](acetyl form) + H2O | Rubrivivax gelatinosus | - |
[citrate (pro-3S)-lyase](thiol form) + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Enterobacter aerogenes | - |
- |
- |
no activity in Streptococcus diacetilactis | - |
- |
- |
Rubrivivax gelatinosus | - |
DSM 149 | - |
Purification (Comment) | Organism |
---|---|
- |
Rubrivivax gelatinosus |
Storage Stability | Organism |
---|---|
-15°C, purified enzyme stable for at least 6 months | Rubrivivax gelatinosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acetyl-CoA, acetoacetyl-CoA, S-acetyl-N-acetylcyseamine and purified citrate lyases from Enterobacter aerogenes and Streptococcus diacetilactis are no substrates, reaction with butyryl-S-citrate lyase, propionyl-S-citrate lyaseand acetyl-S-citrate lyase only slightly faster than spontaneous hydrolysis | Rubrivivax gelatinosus | ? | - |
? | |
[citrate (pro-3S)-lyase](acetyl form) + H2O | - |
Rubrivivax gelatinosus | [citrate (pro-3S)-lyase](thiol form) + acetate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
- |
Rubrivivax gelatinosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.1 | because of the instability of the substrate acetyl-S-citrate lyase at acidic and alkaline pH-values, all assays are carried out at pH 7.2, where the spontanous hydrolysis of the substrate is negligible and the enzyme shows 70% of the activity at pH 8.1 | Rubrivivax gelatinosus |