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Literature summary for 3.1.2.14 extracted from
- Acyl carrier proteins from sunflower (Helianthus annuus L.) seeds and their influence on FatA and FatB acyl-ACP thioesterase activities (2016), Planta, 244, 479-490 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Helianthus annuus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00084 | - |
palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus |  |
| 0.0009 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0009 | 0.002 | oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0011 | - |
stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0013 | - |
palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0013 | - |
oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0021 | - |
oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0021 | 0.0028 | oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.004 | 0.0051 | palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0042 | 0.0049 | stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0045 | 0.0051 | palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.0067 | 0.0128 | stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + H2O | best substrate for isoform FatA | Helianthus annuus | [acyl-carrier protein] + oleate | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | best substrate for isoform FatB | Helianthus annuus | [acyl-carrier protein] + palmitate | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | [acyl-carrier protein] + stearate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-ACP thioesterase | - |
Helianthus annuus |
| FatA | - |
Helianthus annuus |
| FatB | - |
Helianthus annuus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | 0.7 | oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.27 | - |
stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.4 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.5 | - |
palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 0.52 | - |
palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 1.19 | - |
oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.5 | 4.2 | palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 3.66 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 9.4 | 9.6 | palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 11.4 | - |
oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 17.3 | 25.5 | oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 20.4 | 26.5 | stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 90 | 850 | oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 240 | - |
stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 400 | - |
palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 400 | - |
stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 620 | - |
palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 760 | 850 | palmitoyl-[acyl-carrier protein] | isoform FatA, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 810 | 1300 | stearoyl-[acyl-carrier protein] | isoform FatA, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 910 | - |
oleoyl-[acyl-carrier protein] | isoform FatB, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 1900 | 2400 | palmitoyl-[acyl-carrier protein] | isoform FatB, palmitoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 2100 | 3200 | stearoyl-[acyl-carrier protein] | isoform FatB, stearoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 5400 | - |
oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Escherichia coli, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
| 7400 | 12300 | oleoyl-[acyl-carrier protein] | isoform FatA, oleoyl-[acyl-carrier protein] from Helianthus annuus, at pH 8.0, temperature not specified in the publication | Helianthus annuus | |
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