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Literature summary for 3.1.2.14 extracted from

  • Jing, F.; Yandeau-Nelson, M.D.; Nikolau, B.J.
    Identification of active site residues implies a two-step catalytic mechanism for acyl-ACP thioesterase (2018), Biochem. J., 475, 3861-3873 .
    View publication on PubMed
Search for more literature for 3.1.2.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain K27 and BL21 Star (DE3) cells Cuphea viscosissima
expressed in Escherichia coli strain K27 and BL21 Star (DE3) cells Cocos nucifera

Protein Variants

Protein Variants Comment Organism
C348A the mutant of isoform FatB2 shows about 60% of wild type activity Cuphea viscosissima
C348S the mutant of isoform FatB2 shows about 140% of wild type activity Cuphea viscosissima
C352A the mutant of isoform FatB1 shows reduced activity compared to the wild type enzyme Cuphea viscosissima
C352S the mutant of isoform FatB1 shows reduced activity compared to the wild type enzyme Cuphea viscosissima
C358A the mutant shows increased activity compared to the wild type enzyme Cocos nucifera
c358S the mutant shows reduced activity compared to the wild type enzyme Cocos nucifera
D309A the mutant of isoform FatB2 has less than 3% activity compared to the wild type enzyme Cuphea viscosissima
D309E inactive Cuphea viscosissima
D309N the mutant of isoform FatB2 has less than 5% activity compared to the wild type enzyme Cuphea viscosissima
E347A inactive Cuphea viscosissima
E347D inactive Cuphea viscosissima
E347Q inactive Cuphea viscosissima
E351A the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme Cuphea viscosissima
E351D the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme Cuphea viscosissima
E357A the mutant shows strongly reduced activity compared to the wild type enzyme Cocos nucifera
E357D the mutant shows strongly reduced activity compared to the wild type enzyme Cocos nucifera
H313A inactive Cuphea viscosissima
H317A the mutant of isoform FatB1 shows almost no activity compared to the wild type enzyme Cuphea viscosissima
H323A the mutant shows almost no activity compared to the wild type enzyme Cocos nucifera
N311A the mutant of isoform FatB2 has less than 8% activity compared to the wild type enzyme Cuphea viscosissima
N315A the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme Cuphea viscosissima
N321A the mutant shows strongly reduced activity compared to the wild type enzyme Cocos nucifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an oleoyl-[acyl-carrier protein] + H2O Cuphea viscosissima
-
 an [acyl-carrier protein] + oleate
-
 ?
an oleoyl-[acyl-carrier protein] + H2O Cocos nucifera
-
 an [acyl-carrier protein] + oleate
-
 ?

Organism

Organism UniProt Comment Textmining
Cocos nucifera G3ESU7
-
-
Cuphea viscosissima G3ESU9
-
-
Cuphea viscosissima G3ESV0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Cuphea viscosissima
Ni-NTA column chromatography Cocos nucifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an oleoyl-[acyl-carrier protein] + H2O
-
 Cuphea viscosissima an [acyl-carrier protein] + oleate
-
 ?
an oleoyl-[acyl-carrier protein] + H2O
-
 Cocos nucifera an [acyl-carrier protein] + oleate
-
 ?

Synonyms

Synonyms Comment Organism
acyl-ACP TE
-
 Cuphea viscosissima
acyl-ACP TE
-
 Cocos nucifera
acyl-ACP-thioesterase
-
 Cuphea viscosissima
acyl-ACP-thioesterase
-
 Cocos nucifera
acyl-acyl carrier protein thioesterase
-
 Cuphea viscosissima
acyl-acyl carrier protein thioesterase
-
 Cocos nucifera
FATB1 isoform Cuphea viscosissima
FatB2 isoform Cuphea viscosissima
FatB2 isoform Cocos nucifera