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Literature summary for 3.1.2.14 extracted from
- Tuning of acyl-ACP thioesterase activity directed for tailored fatty acid synthesis (2018), Appl. Microbiol. Biotechnol., 102, 3173-3182 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli K27 cells | Cuphea hookeriana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I198E | the mutant has highest activity with decanoyl-[acyl-carrier protein] | Cuphea hookeriana |
| K287E | compared to the wild type enzyme, the mutant has higher activity with decanoyl-[acyl-carrier protein] and lower activity with octanoyl-[acyl-carrier protein] | Cuphea hookeriana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
decanoyl-[acyl-carrier protein] | wild type enzyme, at pH 8.0 and 27°C | Cuphea hookeriana |  |
| 0.29 | - |
decanoyl-[acyl-carrier protein] | mutant enzyme K287E, at pH 8.0 and 27°C | Cuphea hookeriana | |
| 0.39 | - |
decanoyl-[acyl-carrier protein] | mutant enzyme I198E, at pH 8.0 and 27°C | Cuphea hookeriana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| decanoyl-[acyl-carrier protein] + H2O | Cuphea hookeriana | - |
[acyl-carrier protein] + decanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cuphea hookeriana | Q39514 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Cuphea hookeriana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| decanoyl-[acyl-carrier protein] + H2O | - |
Cuphea hookeriana | [acyl-carrier protein] + decanoate | - |
? | |
| nonanoyl-[acyl-carrier protein] + H2O | - |
Cuphea hookeriana | [acyl-carrier protein] + nonanoate | - |
? | |
| octanoyl-[acyl-carrier protein] + H2O | preferred substrate | Cuphea hookeriana | [acyl-carrier protein] + octanoate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FatB2 | C8-C10-specific thioesterase | Cuphea hookeriana |
| medium-chain acyl-ACP thioesterase | - |
Cuphea hookeriana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
decanoyl-[acyl-carrier protein] | mutant enzyme I198E, at pH 8.0 and 27°C | Cuphea hookeriana | |
| 0.24 | - |
decanoyl-[acyl-carrier protein] | mutant enzyme K287E, at pH 8.0 and 27°C | Cuphea hookeriana | |
| 0.33 | - |
decanoyl-[acyl-carrier protein] | wild type enzyme, at pH 8.0 and 27°C | Cuphea hookeriana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
decanoyl-[acyl-carrier protein] | mutant enzyme I198E, at pH 8.0 and 27°C | Cuphea hookeriana | |
| 0.82 | - |
decanoyl-[acyl-carrier protein] | mutant enzyme K287E, at pH 8.0 and 27°C | Cuphea hookeriana | |
| 2.43 | - |
decanoyl-[acyl-carrier protein] | wild type enzyme, at pH 8.0 and 27°C | Cuphea hookeriana | |
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