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Literature summary for 3.1.2.14 extracted from
- Deposition of stearate-oleate rich seed fat in Mangifera indica is mediated by a FatA type acyl-ACP thioesterase (2011), Phytochemistry, 72, 166-177.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
stearoyl-[acyl-carrier protein] | pH 9, 37°C | Mangifera indica |  |
| 0.004 | - |
oleoyl-[acyl-carrier protein] | pH 9, 37°C | Mangifera indica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, Western blot analysis | Mangifera indica |
| 78000 | - |
gel filtration | Mangifera indica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mangifera indica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, ion exchange chromatography (DEAE), [acyl-carrier protein]-affinity chromatography | Mangifera indica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kernel | - |
Mangifera indica | - |
| seed | - |
Mangifera indica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.18 | - |
substrate stearoyl-[acyl-carrier protein], pH 9, 37°C | Mangifera indica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + H2O | - |
Mangifera indica | acyl-carrier protein + oleic acid | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | relative specific activity to oleoyl-[acyl-carrier protein]: 1.8% | Mangifera indica | acyl-carrier protein + palmitic acid | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | relative specific activity to oleoyl-[acyl-carrier protein]: 35% | Mangifera indica | acyl-carrier protein + stearic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 40000, Western blot analysis | Mangifera indica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fatty acyl-ACP thioesterase | - |
Mangifera indica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
around 35-37°C | Mangifera indica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
activity relatively stable at 35°C | Mangifera indica |
| 60 | - |
60% activity lost after 15 min at 60°C, 75% activity lost after 30 min at 60°C | Mangifera indica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Mangifera indica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mangifera indica | chromatofocusing | - |
7 |
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Release 2023.1 (January 2023)
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