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Literature summary for 3.1.2.14 extracted from

  • Wu, P.Z.; Li, J.; Wei, Q.; Zeng, L.; Chen, Y.P.; Li, M.R.; Jiang, H.W.; Wu, G.J.
    Cloning and functional characterization of an acyl-acyl carrier protein thioesterase (JcFATB1) from Jatropha curcas (2009), Tree Physiol., 29, 1299-1305.
    View publication on PubMed
Search for more literature for 3.1.2.14

Application

Application Comment Organism
industry FATB1 can function as a saturated acyl-ACP TE and may potentially modify the seed oil to increase its levels of palmitate Jatropha curcas

Cloned(Commentary)

Cloned (Comment) Organism
coding region (including the chloroplast targeting peptide) of FATB1 cDNA amplified, then ligated into the 2S::GUS plasmid vector, using XbaI/SacI to place the gusA gene behind the seed-specific promoter. The 2S::JcFATB1 construct transferred into Agrobacterium tumefaciens GV3101, and Arabidopsis plants for overexpression Jatropha curcas

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast putative chloroplast transit peptide at the N-terminal Jatropha curcas 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48080
-
 sequence analysis Jatropha curcas

Organism

Organism UniProt Comment Textmining
Jatropha curcas A7XZV1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Jatropha curcas
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leaf
-
 Jatropha curcas
-
root lowest level Jatropha curcas
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seed highest level Jatropha curcas
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stem
-
 Jatropha curcas
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-[acyl-carrier protein] + H2O FATB1 is a palmitoyl-acyl-acyl carrier protein thioesterase. Seed-specific expression of FATB1 in Arabidopsis leads to a 3-4fold increase in the proportion of accumulated palmitate (up to 40.9 mol%) and moderate increases in the proportions of other saturated fatty acids, with associated reductions in the proportions of unsaturated fatty acids. Residues H317 and C352 are essential for catalytic activity Jatropha curcas palmitate + acyl-carrier protein
-
 ?

Subunits

Subunits Comment Organism
? x * 48080, calculated from sequence Jatropha curcas

Synonyms

Synonyms Comment Organism
acyl-ACP TE
-
 Jatropha curcas
acyl-ACP thioesterase
-
 Jatropha curcas
acyl-acyl carrier protein thioesterase
-
 Jatropha curcas
FATB1
-
 Jatropha curcas

pI Value

Organism Comment pI Value Maximum pI Value
Jatropha curcas sequence analysis
-
 6.57

Expression

Organism Comment Expression
Jatropha curcas is most strongly expressed in seeds, in which its expression peaks in late developmental stages up