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Literature summary for 3.1.2.14 extracted from

  • Sanchez-Garcia, A.; Moreno-Perez, A.J.; Muro-Pastor, A.M.; Salas, J.J.; Garces, R.; Martinez-Force, E.
    Acyl-ACP thioesterases from castor (Ricinus communis L.): An enzymatic system appropriate for high rates of oil synthesis and accumulation (2010), Phytochemistry, 71, 860-869.
    View publication on PubMed
Search for more literature for 3.1.2.14

Application

Application Comment Organism
industry contribution of FatA to the synthesis of castor oil Ricinus communis
industry contribution of FatB to the synthesis of castor oil Ricinus communis

Cloned(Commentary)

Cloned (Comment) Organism
PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatA overexpressed in Escherichia coli XL1-Blue Ricinus communis
PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatB overexpressed in Escherichia coli XL1-Blue Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.00042
-
 oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.00054
-
 palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.00054
-
 stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.00056
-
 palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.00067
-
 oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.00086
-
 palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.00088
-
 stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36800
-
 recombinant histidine-tagged FatB, gel filtration Ricinus communis
37520
-
 recombinant histidine-tagged FatA, gel filtration Ricinus communis
42200
-
 FatA open reading frames, calculated from sequence Ricinus communis
46500
-
 FatB open reading frames, calculated from sequence Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis A9XK92
-
-
Ricinus communis A9XUG5
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity in a single step by gel filtration Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Ricinus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives Ricinus communis ?
-
 ?
oleoyl-[acyl-carrier protein] + H2O high efficiency of FatB Ricinus communis oleic acid + acyl-carrier protein
-
 ?
oleoyl-[acyl-carrier protein] + H2O maximum catalytic efficiency of FatA Ricinus communis oleic acid + acyl-carrier protein
-
 ?
palmitoleoyl-[acyl-carrier protein] + H2O high catalytic efficiency of FatA Ricinus communis palmitoleate + acyl-carrier protein
-
 ?
palmitoleoyl-[acyl-carrier protein] + H2O high efficiency of FatB Ricinus communis palmitoleate + acyl-carrier protein
-
 ?
palmitoyl-[acyl-carrier protein] + H2O
-
 Ricinus communis palmitate + acyl-carrier protein
-
 ?
stearoyl-[acyl-carrier protein] + H2O
-
 Ricinus communis stearate + acyl-carrier protein
-
 ?

Subunits

Subunits Comment Organism
? x * 42200, FatA open reading frames, calculated from sequence Ricinus communis
? x * 46500, FatA open reading frames, calculated from sequence Ricinus communis

Synonyms

Synonyms Comment Organism
acyl-ACP thioesterase
-
 Ricinus communis
acyl-acyl carrier protein (ACP) thioesterase
-
 Ricinus communis
acyl–ACP thioesterase
-
 Ricinus communis
acyl–acyl carrier protein (ACP) thioesterase
-
 Ricinus communis
FatA
-
 Ricinus communis
FatB
-
 Ricinus communis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.38
-
 stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.41
-
 palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.46
-
 palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.56
-
 stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
0.67
-
 oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
1.62
-
 palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
7.99
-
 oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis

pI Value

Organism Comment pI Value Maximum pI Value
Ricinus communis calculated from sequence
-
 6.7
Ricinus communis calculated from sequence
-
 7

Expression

Organism Comment Expression
Ricinus communis transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
250
-
 palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
440
-
 stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
760
-
 palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
830
-
 palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
990
-
 oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
1030
-
 stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
4630
-
 palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
19030
-
 oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis