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Literature summary for 3.1.2.12 extracted from
- Purification and properties of S-formylglutathione hydrolase from human liver (1974), J. Biol. Chem., 249, 7664-7672.
General Stability
| General Stability | Organism |
|---|---|
| stabilization by 2-mercaptoethanol or dithiothreitol | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4,6-Trinitrobenzene sulfonate | - |
Homo sapiens |  |
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Homo sapiens | |
| arsenite | - |
Homo sapiens | |
| ascorbate | - |
Homo sapiens | |
| CaCl2 | - |
Homo sapiens | |
| Co2+ | - |
Homo sapiens | |
| CuSO4 | - |
Homo sapiens | |
| F- | - |
Homo sapiens | |
| folate | - |
Homo sapiens | |
| glutathione | - |
Homo sapiens | |
| HgCl2 | - |
Homo sapiens | |
| iodoacetate | - |
Homo sapiens | |
| p-hydroxymercuribenzoate | - |
Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
S-acetylglutathione | - |
Homo sapiens | |
| 0.29 | - |
S-formylglutathione | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Homo sapiens |
| 52000 | - |
gel filtration | Homo sapiens |
| 58200 | - |
calculation from sedimentation and diffusion coefficients | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4050 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-acetylglutathione + H2O | slight activity | Homo sapiens | glutathione + acetate | - |
ir | |
| S-formylglutathione + H2O | - |
Homo sapiens | glutathione + formate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activation energy 4.5 cal/mol | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 7.1 | - |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 8.6 | stable for 30 min at 20°C | Homo sapiens |
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