Cloned (Comment) | Organism |
---|---|
- |
Agrobacterium tumefaciens |
Crystallization (Comment) | Organism |
---|---|
by the sitting drop-vapor diffusion technique, at 2 A resolution. Crystals belong to space group P1 with unit cell dimensions a = 66.43 A, b = 68.95 A, c = 95.65 A, alpha = 92.41, beta = 99.79, gamma = 98.43. Contacts between the two monomers in the dimer are formed both by hydrogen bonds and salt bridges | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
C54S | shows a 4fold decrease in alpha-naphthyl butyrate activity. Is insensitive to the inhibitor N-ethylmaleimide | Agrobacterium tumefaciens |
S147A | is unable to hydrolyse alpha-naphthyl butyrate | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | binding of the inhibitor molecule to the conserved cysteine residue Cys54 located in the active site of the enzyme, consequently, blocking the binding of substrate to the active site | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-nitrophenyl caproate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
0.05 | - |
4-nitrophenyl propionate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
0.11 | - |
4-nitrophenyl butyrate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
0.35 | - |
alpha-naphthyl propionate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
0.44 | - |
naphthalen-1-yl n-butanoate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
0.48 | - |
4-nitrophenyl acetate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | highest activity | Agrobacterium tumefaciens | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + propionate | - |
? | |
alpha-naphthyl butyrate + H2O | - |
Agrobacterium tumefaciens | alpha-naphthol + butyrate | - |
? | |
alpha-naphthyl propionate + H2O | - |
Agrobacterium tumefaciens | alpha-naphthol + propionate | - |
? | |
additional information | is unable to hydrolyse alpha-naphthyl dodecanoate, 4-nitrophenyl caprate, 4-nitrophenyl laurate, and 4-nitrophenyl palmitate. Potential role for Cys54 in regulation of enzyme activity through S-glutathionylation. Hydrolyzes C-O bonds with high affinity toward short to medium chain esters (C3-C4). Has more affinity toward C3 and C4 4-nitrophenyl esters than toward C3 and C4 alpha-naphthyl esters | Agrobacterium tumefaciens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | crystallography | Agrobacterium tumefaciens |
Synonyms | Comment | Organism |
---|---|---|
Atu1476 | - |
Agrobacterium tumefaciens |
S-formylglutathione hydrolase | - |
Agrobacterium tumefaciens |
SFGH | - |
Agrobacterium tumefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
4-nitrophenyl caproate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
1.73 | - |
naphthalen-1-yl n-butanoate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
1.84 | - |
4-nitrophenyl propionate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
1.9 | - |
alpha-naphthyl propionate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
1.95 | - |
4-nitrophenyl acetate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
4.48 | - |
4-nitrophenyl butyrate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.93 | - |
naphthalen-1-yl n-butanoate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
4.07 | - |
4-nitrophenyl acetate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
5.54 | - |
alpha-naphthyl propionate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
38.5 | - |
4-nitrophenyl propionate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
39.6 | - |
4-nitrophenyl caproate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens | |
40.8 | - |
4-nitrophenyl butyrate | in Tris-HCl buffer, pH 8.0 | Agrobacterium tumefaciens |