Any feedback?
Literature summary for 3.1.2.12 extracted from
- Cloning and characterisation of S-formylglutathione hydrolase from Arabidopsis thaliana: a pathway for formaldehyde detoxification (2002), Plant Physiol. Biochem., 40, 281-288.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTNB | 48% inhibition at 0.001 mM | Arabidopsis thaliana |  |
| p-hydroxymercuribenzoate | 59% inhibition at 0.001 mM | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.087 | - |
4-yethylumbelliferyl acetate | pH 7.0, 25°C | Arabidopsis thaliana | |
| 0.318 | - |
S-formylglutathione | pH 7.0, 25°C | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, about, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-formylglutathione + H2O | Arabidopsis thaliana | key enzyme of the formaldehyde detoxification, glutathione recycling | glutathione + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
ecotype Columbia | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.86 | - |
recombinant enzyme, crude enzyme extract | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl acetate + H2O | - |
Arabidopsis thaliana | 4-methylumbelliferol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + acetate | - |
? | |
| additional information | 2-nitrophenyl acetate is a poor substrate | Arabidopsis thaliana | ? | - |
? | |
| S-formylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + formate | - |
? | |
| S-formylglutathione + H2O | key enzyme of the formaldehyde detoxification, glutathione recycling | Arabidopsis thaliana | glutathione + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, about, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FGH | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
