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Literature summary for 3.1.2.12 extracted from
- Cloning and characterization of an S-formylglutathione hydrolase from Arabidopsis thaliana (2002), Arch. Biochem. Biophys., 399, 232-238.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid | Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determinationand analysis, expression in Escherichia coli as C-terminally His-tagged enzyme | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 90% reduced activity at 0.01 mM | Arabidopsis thaliana |  |
| additional information | no inhibition by PMSF, not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid | Arabidopsis thaliana | |
| N-ethylmaleimide | complete inhibition at 1 mM | Arabidopsis thaliana | |
| Zn2+ | 50% reduced activity at 0.01 mM | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
fluorescein diacetate | recombinant enzyme, pH 7.2, 37°C | Arabidopsis thaliana | |
| 0.12 | - |
4-yethylumbelliferyl acetate | recombinant enzyme, pH 7.5, 37°C | Arabidopsis thaliana | |
| 0.13 | - |
S-formylglutathione | recombinant enzyme, pH 7.2, 37°C | Arabidopsis thaliana | |
| 0.15 | - |
S-acetylglutathione | recombinant enzyme, pH 7.2, 37°C | Arabidopsis thaliana | |
| 0.54 | - |
beta-naphthyl acetate | recombinant enzyme, pH 7.2, 37°C | Arabidopsis thaliana | |
| 0.57 | - |
Alpha-naphthyl acetate | recombinant enzyme, pH 7.2, 37°C | Arabidopsis thaliana | |
| 1.02 | - |
4-nitrophenyl acetate | recombinant enzyme, pH 7.2, 37°C | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31700 | - |
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag | Arabidopsis thaliana |
| 66000 | - |
recombinant enzyme, gel filtration | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-formylglutathione + H2O | Arabidopsis thaliana | enzyme is involved in formaldehyde detoxification | glutathione + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Arabidopsis thaliana | - |
| plant | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.08 | - |
recombinant enzyme, substrate fluorescein diacetate | Arabidopsis thaliana |
| 1.3 | - |
recombinant enzyme, substrate beta-naphthyl acetate | Arabidopsis thaliana |
| 5.7 | - |
recombinant enzyme, substrate 4-nitrophenyl acetate | Arabidopsis thaliana |
| 10.4 | - |
recombinant enzyme, substrate S-formylglutathione | Arabidopsis thaliana |
| 14.5 | - |
recombinant enzyme, substrate S-acetylglutathione | Arabidopsis thaliana |
| 15.2 | - |
recombinant enzyme, substrate alpha-naphthyl acetate | Arabidopsis thaliana |
| 36.3 | - |
recombinant enzyme, substrate 4-methylumbelliferyl acetate | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl acetate + H2O | - |
Arabidopsis thaliana | 4-methylumbelliferol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + acetate | - |
? | |
| alpha-naphthyl acetate + H2O | - |
Arabidopsis thaliana | alpha-naphthol + acetate | - |
? | |
| beta-naphthyl acetate + H2O | - |
Arabidopsis thaliana | beta-naphthol + acetate | - |
? | |
| fluorescein diacetate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| S-acetylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + acetate | - |
? | |
| S-formylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + formate | - |
? | |
| S-formylglutathione + H2O | enzyme is involved in formaldehyde detoxification | Arabidopsis thaliana | glutathione + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SFGH | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
fully stable up to, rapid inactivation at higher temperatures | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at, except with substrate 4-methylumbelliferyl acetate | Arabidopsis thaliana |
| 7.6 | 8 | optimum | Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | isoelectric focusing | - |
6 |
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