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Literature summary for 3.1.2.12 extracted from

  • McAuley, K.E.; Cummins, I.; Papiz, M.; Edwards, R.; Fordham-Skelton, A.P.
    Purification, crystallization and preliminary X-ray diffraction analysis of S-formylglutathione hydrolase from Arabidopsis thaliana: effects of pressure and selenomethionine substitution on space-group changes (2003), Acta Crystallogr. Sect. D, 59, 2272-2274.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression as His-tagged enzyme in Escherichia coli BL21(DE3), production of a selenomethionine labelled enzyme in a methionine auxotrophic strain Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
selenomethionine labelled recombinant enzyme, 30 mg/ml, hanging-drop vapor-diffusion method, 293K, precipitant solution: 16% PEG 4000, 0.2 M magnesium acetate, 0.2 M imidazole-malate, pH 6.5, 3% methanol, optimal crystals by microseeding, 1-5 d, production of selenomethionine-enzyme crystals is similar with 10% PEG 4000 and additional presence of 10 mM DTT, X-ray diffraction structure determination and analysis at 1.84 A resolution Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-formylglutathione + H2O Arabidopsis thaliana key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling glutathione + formate
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Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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-
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Purification (Commentary)

Purification (Comment) Organism
recombinant from overexpressing Escherichia coli BL21(DE3) as His-tagged enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-formylglutathione + H2O
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Arabidopsis thaliana glutathione + formate
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?
S-formylglutathione + H2O key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling Arabidopsis thaliana glutathione + formate
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?

Synonyms

Synonyms Comment Organism
SFGH
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Arabidopsis thaliana