Cloned (Comment) | Organism |
---|---|
expression as His-tagged enzyme in Escherichia coli BL21(DE3), production of a selenomethionine labelled enzyme in a methionine auxotrophic strain | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
selenomethionine labelled recombinant enzyme, 30 mg/ml, hanging-drop vapor-diffusion method, 293K, precipitant solution: 16% PEG 4000, 0.2 M magnesium acetate, 0.2 M imidazole-malate, pH 6.5, 3% methanol, optimal crystals by microseeding, 1-5 d, production of selenomethionine-enzyme crystals is similar with 10% PEG 4000 and additional presence of 10 mM DTT, X-ray diffraction structure determination and analysis at 1.84 A resolution | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-formylglutathione + H2O | Arabidopsis thaliana | key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling | glutathione + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from overexpressing Escherichia coli BL21(DE3) as His-tagged enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-formylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + formate | - |
? | |
S-formylglutathione + H2O | key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling | Arabidopsis thaliana | glutathione + formate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SFGH | - |
Arabidopsis thaliana |