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Literature summary for 3.1.2.1 extracted from
- On the regulation of cold-labile cytosolic and of mitochondrial acetyl-CoA hydrolase in rat liver (1985), Eur. J. Biochem., 147, 111-117.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 5'-[beta,gamma-imido]triphosphate | with adoPP[CH2]P a 2.5fold activation of the cold-labile cytosolic enzyme at 2 mM, with adoPP[NH]P a 3.7fold activation respectively | Rattus norvegicus |  |
| ATP | 14fold activation of the cold-labile enzyme at 2 mM | Rattus norvegicus | |
| CTP | counteracts the inhibition of the cold-labile cytosolic enzyme by ADP | Rattus norvegicus | |
| GTP | 4.6fold activation of the cold-labile cytosolic enzyme at 2 mM | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 50% inhibition of the cold-labile cytosolic enzyme at 0.0145 mM | Rattus norvegicus | |
| ATP | 0.5 mM nucleoside phosphate inhibits 49% in Trypanosoma brucei brucei 427 | Rattus norvegicus | |
| CoA | 80% inhibition of the cold-labile cytosolic enzyme at 0.04 mM | Rattus norvegicus | |
| GDP | 50% inhibition of the cold-labile cytosolic enzyme at 0.0145 mM | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
SDS-PAGE gel electrophoresis, the cold labile cytosolic acetyl-CoA hydrolase | Rattus norvegicus |
| 136000 | - |
gel filtration, cytosolic native enzyme in the presence of 2 mM ATP, dimeric form | Rattus norvegicus |
| 157000 | - |
gel filtration, mitochondrial enzyme | Rattus norvegicus |
| 245000 | - |
gel filtration, cytosolic native enzyme in the presence of 2 mM ATP, tetrameric form | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O | Rattus norvegicus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O | - |
Rattus norvegicus | CoA + acetate | - |
? | |
| acetyl-CoA + H2O | - |
Rattus norvegicus | ? | - |
? | |
| butyryl-CoA + H2O | - |
Rattus norvegicus | butanoate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Rattus norvegicus |
| tetramer | - |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0145 | - |
GDP | cold-labile cytosolic enzyme | Rattus norvegicus | |
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