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Literature summary for 3.1.2.1 extracted from
- A cold-labile acetyl-coenzyme-A hydrolase from the supernatant fraction of rat liver. Reactivation and reconstitution of the active species from the inactive monomer (1984), Eur. J. Biochem., 142, 177-181.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | at time of rewarming 0.5 mM acetyl-CoA restores the activity about 50% | Rattus norvegicus |  |
| albumin | albumin enhances the reactivation of the monomeric form | Rattus norvegicus | |
| ATP | at time of rewarming ATP greatly enhances the restoration of the activity, 2 mM ATP restores the enzyme activity about 70% | Rattus norvegicus | |
| diphosphate | 450 mM pyrophosphate restores the activity about 80% at time of rewarming | Rattus norvegicus | |
| phosphate | 920 mM phosphate restores the activity about 80% at time of rewarming | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | extramitochondrial | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 135000 | - |
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| from cold inactivation | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | sucrose density gradient centrifugation | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
warming at 37°C after cold exposure is optimal for reactivation | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | purified dimeric enzyme fairly stable in potassium phosphate buffer of pH 7.0-8.0 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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