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Literature summary for 3.1.2.1 extracted from
- Molecular cloning and functional expression of rat liver cytosolic acetyl-CoA hydrolase (2001), Eur. J. Biochem., 268, 2700-2709.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Sf9 cells | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.153 | - |
acetyl-CoA | 25°C, recombinant enzyme | Rattus norvegicus |  |
| 0.22 | - |
acetyl-CoA | 25°C, native enzyme | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
2 * 62000, SDS-PAGE | Rattus norvegicus |
| 62017 | - |
2 * 62017, calculation from nucleotide sequence | Rattus norvegicus |
| 124000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | Q99NB7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 329 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O | - |
Rattus norvegicus | CoA + acetate | - |
? | |
| butanoyl-CoA + H2O | about 20% of the activity with acetyl-CoA | Rattus norvegicus | CoA + butanoate | - |
? | |
| hexanoyl-CoA + H2O | about 10% of the activity with acetyl-CoA | Rattus norvegicus | CoA + hexanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, SDS-PAGE | Rattus norvegicus |
| dimer | 2 * 62017, calculation from nucleotide sequence | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CACH | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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