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Literature summary for 3.1.15.1 extracted from

  • Al-Saleh, S.S.; Khan, S.
    Purification and characterization of phosphodiesterase I from Walterinnesia aegyptia venom (2011), Prep. Biochem. Biotechnol., 41, 262-277.
    View publication on PubMed
Search for more literature for 3.1.15.1

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive inhibition Walterinnesia aegyptia
cysteine noncompetitive inhibition Walterinnesia aegyptia
EDTA strong inhibition, addition of 10 mM Ca2+ restored only 8% of the enzyme activity Walterinnesia aegyptia
glutathione
-
 Walterinnesia aegyptia
o-phenanthroline
-
 Walterinnesia aegyptia

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Walterinnesia aegyptia
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 13% activation at 15-20 mM Walterinnesia aegyptia
additional information the enzyme is active in the absence of any divalent ions and the addition of Ca2+ or Mg2+ has no significant effect on the activity of Walterinnesia aegyptia PDE Walterinnesia aegyptia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
158000
-
 x * 158000, SDS-PAGE Walterinnesia aegyptia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Walterinnesia aegyptia the enzyme sequentially removes mononucleotide units from the polynucleotide chain starting from the free 3' hydroxyl end, and can attack DNA, RNA, or any nucleotide chain ?
-
 ?

Organism

Organism UniProt Comment Textmining
Walterinnesia aegyptia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 3fold from venom by preparative native polyacrylamide gel electrophoresis Walterinnesia aegyptia

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Walterinnesia aegyptia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
 purified native enzyme, pH 9.0, 37°C Walterinnesia aegyptia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Walterinnesia aegyptia ?
-
 ?
cAMP + H2O very low activity Walterinnesia aegyptia ?
-
 ?
additional information the enzyme sequentially removes mononucleotide units from the polynucleotide chain starting from the free 3' hydroxyl end, and can attack DNA, RNA, or any nucleotide chain Walterinnesia aegyptia ?
-
 ?
thymidine-5'-phosphate 4-nitrophenyl ester + H2O
-
 Walterinnesia aegyptia ?
-
 ?

Subunits

Subunits Comment Organism
? x * 158000, SDS-PAGE Walterinnesia aegyptia

Synonyms

Synonyms Comment Organism
PDE-I
-
 Walterinnesia aegyptia
venom exonuclease I
-
 Walterinnesia aegyptia
venom PDE-I
-
 Walterinnesia aegyptia
venom phosphodiesterase I
-
 Walterinnesia aegyptia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Walterinnesia aegyptia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 assay range, rapid loss of activity above 65°C Walterinnesia aegyptia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9 assay at, dependent on the substrate Walterinnesia aegyptia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.8
-
 ADP pH pH 9.0, 37°C Walterinnesia aegyptia
6.2
-
 cysteine pH pH 9.0, 37°C Walterinnesia aegyptia

pI Value

Organism Comment pI Value Maximum pI Value
Walterinnesia aegyptia isoelectric focusing
-
 7.48

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.6
-
 pH pH 9.0, 37°C Walterinnesia aegyptia cysteine
8.3
-
 pH pH 9.0, 37°C Walterinnesia aegyptia ADP