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Literature summary for 3.1.14.1 extracted from

  • Shetty, J.K.; Weaver, R.C.; Kinsella, J.E.
    Ribonuclease isolated from yeast (Saccharomyces carlsbergensis): characterization and properties (1981), Biotechnol. Bioeng., 23, 953-964.
No PubMed abstract available

Application

Application Comment Organism
nutrition possibility for reducing nucleic acid content in yeast protein used in human food Saccharomyces pastorianus

Inhibitors

Inhibitors Comment Organism Structure
1,4-dithiothreitol 50% loss of activity at 5 mM, 75% at 10 mM Saccharomyces pastorianus
Ca2+ 77% of activity at 1 mM CaCl2, 73% at 10 mM Saccharomyces pastorianus
Cu2+ 73% of activity at 1 mM CuCl2, no activity at 10 mM Saccharomyces pastorianus
Fe2+ 76% of activity at 1 mM FeCl2, 46% at 10 mM Saccharomyces pastorianus
Mg2+ 91% of activity at 1 mM MgCl2, 77% at 10 mM Saccharomyces pastorianus
additional information tryptophan residues and disulfide bonds required for activity Saccharomyces pastorianus
N-bromosuccinimide
-
Saccharomyces pastorianus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 125% of activity at 0.05 M, 138% at 0.5 M Saccharomyces pastorianus
NaCl 112% of activity at 0.05 M, 125% at 0.5 M Saccharomyces pastorianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleic acid + H2O Saccharomyces pastorianus exonucleolytic cleavage 3'-phosphomononucleotides
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces pastorianus
-
-
-

Oxidation Stability

Oxidation Stability Organism
disulfide bonds required for activity Saccharomyces pastorianus

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces pastorianus

Reaction

Reaction Comment Organism Reaction ID
exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Saccharomyces pastorianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides
-
?
ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?

Synonyms

Synonyms Comment Organism
RNase
-
Saccharomyces pastorianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
Saccharomyces pastorianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
NaCl and KCl might protect from heat denaturation at 52°C Saccharomyces pastorianus
50
-
10 min, less than 10% loss of activity Saccharomyces pastorianus
70
-
2 min, complete inactivation Saccharomyces pastorianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Saccharomyces pastorianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 at 2°C Saccharomyces pastorianus
5 10 at 40°C: inactivation rate at pH 10 markedly higher than at pH 4.1, 6.3 and 8.6 Saccharomyces pastorianus