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Literature summary for 3.1.13.4 extracted from

  • Schröder, H.C.; Zahn, R.K.; Dose, K.; Muller, W.E.G.
    Purification and characterization of a poly(A)-specific exoribonuclease from calf thymus (1980), J. Biol. Chem., 255, 4535-4538.
    View publication on PubMed
Search for more literature for 3.1.13.4

General Stability

General Stability Organism
stabilization with bovine serum albumin Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide
-
 Bos taurus
NaCl inhibition at 0.2 M ionic strength; inhibits at 150-200 mM Bos taurus
o-Iodobenzoate
-
 Bos taurus
phosphate inhibits at 10 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
 Poly(A)
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Bos taurus
Ba2+ less effective than Mg2+ or Mn2+ Bos taurus
Ca2+ activates Bos taurus
Ca2+ less effective than Mg2+ or Mn2+ Bos taurus
Co2+ activates Bos taurus
Co2+ less effective than Mg2+ or Mn2+ Bos taurus
Cu2+ activates Bos taurus
Cu2+ less effective than Mg2+ or Mn2+ Bos taurus
Mg2+ required Bos taurus
Mg2+ optimal activity at 3 mM Bos taurus
Mg2+ can be replaced by Mn2+ Bos taurus
Mn2+ required Bos taurus
Mn2+ optimal activity at 0.5 mM Bos taurus
Mn2+ can be replaced by Mg2+ Bos taurus
Sn2+ activates Bos taurus
Sn2+ less effective than Mg2+ or Mn2+ Bos taurus
Zn2+ activates Bos taurus
Zn2+ less effective than Mg2+ or Mn2+ Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 1 * 58000, 1 * 31000, SDS-PAGE Bos taurus
58000
-
 1 * 58000, 1 * 31000, SDS-PAGE Bos taurus
80000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(A) RNA + H2O Bos taurus no activity on poly(U), poly(C), 23rRNA 5'-AMP + ?
-
 ?
poly(A) RNA + H2O Bos taurus exonucleolytic cleavage of poly(A) in either the single- or double-stranded form (poly(A)*poly(U)) to 5'-AMP (3'-exonucleolytic activity) 5'-AMP + ?
-
 ?
poly(A) RNA + H2O Bos taurus does not disintegrate polyribosomes isolated from quail oviduct 5'-AMP + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0625
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-20oC, 20% glycerol, bovine serum albumin, 2 months, 20% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5-linked oligoadenylates + H2O
-
 Bos taurus 5'-AMP
-
 ?
poly(A) + H2O
-
 Bos taurus AMP
-
 ?
poly(A) RNA + H2O no activity on poly(U), poly(C), 23rRNA Bos taurus 5'-AMP + ?
-
 ?
poly(A) RNA + H2O exonucleolytic cleavage of poly(A) in either the single- or double-stranded form (poly(A)*poly(U)) to 5'-AMP (3'-exonucleolytic activity) Bos taurus 5'-AMP + ?
-
 ?
poly(A) RNA + H2O does not disintegrate polyribosomes isolated from quail oviduct Bos taurus 5'-AMP + ?
-
 ?
poly(A)-mRNA + H2O in single- or double-stranded form Bos taurus 5'-AMP
-
 ?
poly(A)-poly(U) + H2O double-stranded form Bos taurus 5'-AMP + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 58000, 1 * 31000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
39
-
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7 9.4
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
8.4 10.5 pH 8.4: about 25% of maximum activity, pH 10.5: about 15% of maximum activity Bos taurus