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Literature summary for 3.1.13.3 extracted from

  • Zhou, J.; Zheng, Y.; Roembke, B.; Robinson, S.; Opoku-Temeng, C.; Sayre, D.; Sintim, H.
    Fluorescent analogs of cyclic and linear dinucleotides as phosphodiesterase and oligoribonuclease activity probes (2017), RSC Adv., 7, 5421-5426 .
No PubMed abstract available
Search for more literature for 3.1.13.3

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 50 mM Escherichia coli
EDTA complete inhibition at 50 mM Mycolicibacterium smegmatis
EDTA complete inhibition at 50 mM Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-phosphoguanylyl-(3',5')-guanosine + H2O Escherichia coli i.e. pGpG GMP + guanosine
-
 ?
5'-phosphoguanylyl-(3',5')-guanosine + H2O Pseudomonas aeruginosa i.e. pGpG GMP + guanosine
-
 ?
5'-phosphoguanylyl-(3',5')-guanosine + H2O Mycolicibacterium smegmatis i.e. pGpG GMP + guanosine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A784
-
-
Mycolicibacterium smegmatis A0A8B4R3D6
-
-
Pseudomonas aeruginosa A0A069Q255
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,N6-etheno-2'-dApG + H2O
-
 Escherichia coli 1,N6-etheno-2'-deoxyadenosine + GMP
-
 ?
1,N6-etheno-2'-dApG + H2O
-
 Pseudomonas aeruginosa 1,N6-etheno-2'-deoxyadenosine + GMP
-
 ?
1,N6-etheno-2'-dApG + H2O
-
 Mycolicibacterium smegmatis 1,N6-etheno-2'-deoxyadenosine + GMP
-
 ?
5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Escherichia coli GMP + guanosine
-
 ?
5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Pseudomonas aeruginosa GMP + guanosine
-
 ?
5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Mycolicibacterium smegmatis GMP + guanosine
-
 ?
dApG + H2O
-
 Escherichia coli 2'-deoxyadenosine + GMP
-
 ?
dApG + H2O
-
 Pseudomonas aeruginosa 2'-deoxyadenosine + GMP
-
 ?
dApG + H2O
-
 Mycolicibacterium smegmatis 2'-deoxyadenosine + GMP
-
 ?
additional information poor activity with 4-nitrophenyl phosphate Escherichia coli ?
-
 ?
additional information poor activity with 4-nitrophenyl phosphate Pseudomonas aeruginosa ?
-
 ?
additional information poor activity with 4-nitrophenyl phosphate Mycolicibacterium smegmatis ?
-
 ?

Synonyms

Synonyms Comment Organism
oligoribonuclease
-
 Escherichia coli
oligoribonuclease
-
 Pseudomonas aeruginosa
oligoribonuclease
-
 Mycolicibacterium smegmatis
ORN
-
 Escherichia coli
ORN
-
 Pseudomonas aeruginosa
ORN
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Pseudomonas aeruginosa
37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli
8
-
 assay at Pseudomonas aeruginosa
8
-
 assay at Mycolicibacterium smegmatis

General Information

General Information Comment Organism
physiological function oligoribonuclease (Orn), which had earlier been established as an essential enzyme in most bacteria involved in the recycling of RNA into ribonucleotides, acts as c-di-GMP PDB-B in Pseudomonas aeruginosa cleaving diguanylate into (pGpG), and pGpG then into GMP Pseudomonas aeruginosa