Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Moloney murine leukemia virus |
expressed in Escherichia coli | Human immunodeficiency virus 1 |
expressed in Escherichia coli | Xenotropic MuLV-related virus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 27% (w/v) polyethylene glycol 1500 and 0.1 M sodium citrate, pH 4.7 | Xenotropic MuLV-related virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | potent inhibitor | Human immunodeficiency virus 1 | |
4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | potent inhibitor | Moloney murine leukemia virus | |
4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | potent inhibitor | Xenotropic MuLV-related virus | |
7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | - |
Human immunodeficiency virus 1 | |
7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | - |
Moloney murine leukemia virus | |
7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | - |
Xenotropic MuLV-related virus | |
beta-thujaplicinol | - |
Human immunodeficiency virus 1 | |
beta-thujaplicinol | - |
Moloney murine leukemia virus | |
beta-thujaplicinol | able to inhibit RNase H in the presence of 200 nM HTS-1 RNA-DNA substrate. However, beta-thujaplicinol is not able to inhibit RNase H as the concentration of RNA-DNA substrate is increased | Xenotropic MuLV-related virus | |
trihydroxybenzoylbiphenyl carboxylate hydrazone | - |
Human immunodeficiency virus 1 | |
trihydroxybenzoylbiphenyl carboxylate hydrazone | - |
Moloney murine leukemia virus | |
trihydroxybenzoylbiphenyl carboxylate hydrazone | - |
Xenotropic MuLV-related virus | |
trihydroxybenzoylnaphthyl hydrazone | - |
Human immunodeficiency virus 1 | |
trihydroxybenzoylnaphthyl hydrazone | - |
Moloney murine leukemia virus | |
trihydroxybenzoylnaphthyl hydrazone | - |
Xenotropic MuLV-related virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | RNase H activity is slightly faster in the presence of Mg2+ than Mn2+ | Human immunodeficiency virus 1 | |
Mn2+ | optimal RNase H activitiy in the presence of Mn2+ and not Mg2+ | Moloney murine leukemia virus | |
Mn2+ | optimal RNase H activitiy in the presence of Mn2+ and not Mg2+ | Xenotropic MuLV-related virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Moloney murine leukemia virus | - |
- |
- |
Xenotropic MuLV-related virus | A1Z651 | clone VP62 | - |
Purification (Comment) | Organism |
---|---|
GST column chromatography and gel filtration | Moloney murine leukemia virus |
GST column chromatography and gel filtration | Human immunodeficiency virus 1 |
GST column chromatography and gel filtration | Xenotropic MuLV-related virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | a Cy3-Tr35/Pd22 RNA-DNA hybrid is cut at approximately 18 base pair upstream from the 3' primer end | Human immunodeficiency virus 1 | ? | - |
? | |
DNA-RNA hybrid + H2O | a Cy3-Tr35/Pd22 RNA-DNA hybrid is cut at approximately 19 base pair upstream from the 3' primer end | Moloney murine leukemia virus | ? | - |
? | |
DNA-RNA hybrid + H2O | a Cy3-Tr35/Pd22 RNA-DNA hybrid is cut at approximately 19 base pair upstream from the 3' primer end | Xenotropic MuLV-related virus | ? | - |
? | |
HTS-1 RNA-DNA + H2O | - |
Moloney murine leukemia virus | ? | - |
? | |
HTS-1 RNA-DNA + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
HTS-1 RNA-DNA + H2O | - |
Xenotropic MuLV-related virus | ? | - |
? | |
HTS-2 RNA-DNA + H2O | - |
Moloney murine leukemia virus | ? | - |
? | |
HTS-2 RNA-DNA + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
HTS-2 RNA-DNA + H2O | - |
Xenotropic MuLV-related virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H | - |
Moloney murine leukemia virus |
RNase H | - |
Human immunodeficiency virus 1 |
RNase H | - |
Xenotropic MuLV-related virus |