Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Avian myeloblastosis virus |
expressed in Escherichia coli | Moloney murine leukemia virus |
Protein Variants | Comment | Organism |
---|---|---|
D224A | two Moloney murine leukemia virus reverse transcriptase variants (named MRT-D224A and MRT-D524A) as a negative control, in which the catalytically important residue for the reverse transcription activity, Asp224 and that for the RNase H activity, Asp524, are substituted with Ala, respectively | Moloney murine leukemia virus |
D524A | two Moloney murine leukemia virus reverse transcriptase variants (named MRT-D224A and MRT-D524A) as a negative control, in which the catalytically important residue for the reverse transcription activity, Asp224 and that for the RNase H activity, Asp524, are substituted with Ala, respectively | Moloney murine leukemia virus |
additional information | design of four Moloney murine leukaemia virus/Avian myeloblastosis virus chimeric reverse transcriptases (named MRT-AF, MRT-AP, MRT-AT and MRT-AR) | Avian myeloblastosis virus |
additional information | design of four Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases (named MRT-AF, MRT-AP, MRT-AT and MRT-AR) | Moloney murine leukemia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value of Avian myeloblastosis virus reverse transcriptase (RNase H reaction) above 25 microM. KM-values for different Moloney murine leukaemia virus/Avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 0.013 mM for MRT-AF, 0.0070 mM for MRT-AP, 0.0089 mM for MRT-AT, and 0.0043 mM for MRT-AR | Avian myeloblastosis virus | |
additional information | - |
additional information | Km value of Moloney murine leukemia virus reverse transcriptase (RNase H reaction) above 25 microM. Km values for different Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 0.013 mM for MRT-AF, 0.0070 mM for MRT-AP, 0.0089 mM for MRT-AT, and 0.0043 mM for MRT-AR | Moloney murine leukemia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
single band with a molecular mass of 75 kDa for Moloney murine leukemia virus reverse transcriptase and the mutants MRT-D224A and MRT-D524A, SDS-PAGE. Major single bands with molecular masses in the range of 65-73 kDa, each corresponding to their calculated molecular masses (70203, 71342, 71973 and 66387 Da), respectively, for the Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases (MRT-AF, MRT-AP, MRT-AT, and MRT-AR) | Moloney murine leukemia virus |
additional information | - |
two bands with molecular masses of 95 kDa and 63 kDa for Avian myeloblastosis virus reverse transcriptase (purified from Avian myeloblastosis virus), SDS-PAGE. Major single bands with molecular masses in the range of 65-73 kDa, each corresponding to their calculated molecular masses (70203, 71342, 71973 and 66387 Da), respectively, for the Moloney murine leukaemia virus/Avian myeloblastosis virus chimeric reverse transcriptases (MRT-AF, MRT-AP, MRT-AT, and MRT-AR) | Avian myeloblastosis virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Avian myeloblastosis virus | RNase H activity is determined by measuring the [3H]oligo(rA) released from [3H]poly(rA)*p(dT)45 | ? | - |
? | |
additional information | Moloney murine leukemia virus | RNase H activity is determined by measuring the [3H]oligo(rA) released from [3H]poly(rA)*p(dT)45 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avian myeloblastosis virus | - |
- |
- |
Moloney murine leukemia virus | P03355 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Avian myeloblastosis virus |
- |
Moloney murine leukemia virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of Avian myeloblastosis virus reverse transcriptase at 37°C is 192000 U/mg RNase H, specific activity of the Moloney murine leukaemia virus/Avian myeloblastosis virus chimeric reverse transcriptases: MRT-AF = 10000 U/mg RNase H, MRT-AP U/mg = 10000 U/mg RNase H, MRT-AT U/mg = 13000 U/mg RNase H, and MRT-AR U/mg = 11000 U/mg RNase H. One unit (U) is defined as the amount which produces 1 pmol oligo(rA) (the amount is expressed as that of the total nucleotide) of 10%(w/v) trichloroacetic acid-soluble RNA in 20 min | Avian myeloblastosis virus |
additional information | - |
specific activity of Moloney murine leukemia virus reverse transcriptase at 37°C is 53000 U/mg RNase H, specific activity of the MRT-D224A mutant = 42000 U/mg RNase H, specific activity of the Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases: MRT-AF = 10000 U/mg RNase H, MRT-AP U/mg = 10000 U/mg RNase H, MRT-AT U/mg = 13000 U/mg RNase H, and MRT-AR U/mg = 11000 U/mg RNase H. One unit (U) is defined as the amount which produces 1 pmol oligo(rA) (the amount is expressed as that of the total nucleotide) of 10%(w/v) TCA-soluble RNA in 20 min | Moloney murine leukemia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNase H activity is determined by measuring the [3H]oligo(rA) released from [3H]poly(rA)*p(dT)45 | Avian myeloblastosis virus | ? | - |
? | |
additional information | RNase H activity is determined by measuring the [3H]oligo(rA) released from [3H]poly(rA)*p(dT)45 | Moloney murine leukemia virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H | domain of reverse transcriptase | Avian myeloblastosis virus |
RNase H | domain of reverse transcriptase | Moloney murine leukemia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Avian myeloblastosis virus reverse transcriptase shows a curve with the optimal temperature range of around 44-50°C | Avian myeloblastosis virus |
44 | 50 | Moloney murine leukemia virus reverse transcriptase shows a curve with the optimal temperature range of around 44-50°C | Moloney murine leukemia virus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
RNase H activities of Moloney murine leukemia virus reverse transcriptase and the chimeric reverse transcriptases at various temperatures ranging 30-60°C | Moloney murine leukemia virus |
44 | 50 | RNase H activities of Avian myeloblastosis virus reverse transcriptase and the chimeric reverse transcriptases at various temperatures ranging 30-60°C | Avian myeloblastosis virus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
all chimeric reverse transcriptases are more thermally stable than Avian myeloblastosis virus reverse transcriptase in the RNase H reaction | Avian myeloblastosis virus |
additional information | - |
all chimeric reverse transcriptases are more thermally stable than Moloney murine leukemia virus reverse transcriptase in the RNase H reaction | Moloney murine leukemia virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat value of Avian myeloblastosis virus reverse transcriptase (RNase H reaction) is above 0.025 s-1. The kcat values for different Moloney murine leukaemia virus/Avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 6.3 1/s for MRT-AF, 3.8 1/s for MRT-AP, 4.6 1/s for MRT-AT, and 2.4 1/s for MRT-AR | Avian myeloblastosis virus | |
additional information | - |
additional information | kcat value of Moloney murine leukemia virus reverse transcriptase (RNase H reaction) above 3 ms-1. The kcat values for different Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 6.3 1/s for MRT-AF, 3.8 1/s for MRT-AP, 4.6 1/s for MRT-AT, and 2.4 1/s for MRT-AR | Moloney murine leukemia virus |