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Literature summary for 3.1.13.2 extracted from

  • Kissel, J.D.; Held, D.M.; Hardy, R.W.; Burke, D.H.
    Single-stranded DNA aptamer RT1t49 inhibits RT polymerase and RNase H functions of HIV type 1, HIV type 2, and SIVCPZ RTs (2007), AIDS Res. Hum. Retroviruses, 23, 699-708.
    View publication on PubMed
Search for more literature for 3.1.13.2

Inhibitors

Inhibitors Comment Organism Structure
ddCTP
-
 Human immunodeficiency virus 1
efavirenz
-
 Human immunodeficiency virus 1
nevirapine
-
 Human immunodeficiency virus 1
single-stranded DNA aptamer RT1t49 inhibits viral RT polymerase and RNase H functions, pre-steady-state and order-of-addition kinetic analyses, ssDNA aptamer competes with primer-template for access to RT, and that addition of a nucleoside analog RT inhibitor to the in vitro reaction enhanced the overall effectiveness of both drugs, while nonnucleoside analog RT inhibitors exhibited simple additivity Human immunodeficiency virus 1
single-stranded DNA aptamer RT1t49 inhibits viral RT polymerase and RNase H functions, pre-steady-state and order-of-addition kinetic analyses, ssDNA aptamer competes with primer-template for access to RT, and that addition of a nucleoside analog RT inhibitor to the in vitro reaction enhanced the overall effectiveness of both drugs, while nonnucleoside analog RT inhibitors exhibited simple additivity Human immunodeficiency virus 2
single-stranded DNA aptamer RT1t49 inhibits viral RT polymerase and RNase H functions, pre-steady-state and order-of-addition kinetic analyses, ssDNA aptamer competes with primer-template for access to RT, and that addition of a nucleoside analog RT inhibitor to the in vitro reaction enhanced the overall effectiveness of both drugs, while nonnucleoside analog RT inhibitors exhibited simple additivity Simian immunodeficiency virus
single-stranded RNA aptamer RT1t49 inhibits viral RT polymerase and RNase H functions, pre-steady-state and order-of-addition kinetic analyses, ssDNA aptamer competes with primer-template for access to RT, and that addition of a nucleoside analog RT inhibitor to the in vitro reaction enhanced the overall effectiveness of both drugs, while nonnucleoside analog RT inhibitors exhibited simple additivity Human immunodeficiency virus 1
single-stranded RNA aptamer RT1t49 inhibits viral RT polymerase and RNase H functions, pre-steady-state and order-of-addition kinetic analyses, ssDNA aptamer competes with primer-template for access to RT, and that addition of a nucleoside analog RT inhibitor to the in vitro reaction enhanced the overall effectiveness of both drugs, while nonnucleoside analog RT inhibitors exhibited simple additivity Human immunodeficiency virus 2
single-stranded RNA aptamer RT1t49 inhibits viral RT polymerase and RNase H functions, pre-steady-state and order-of-addition kinetic analyses, ssDNA aptamer competes with primer-template for access to RT, and that addition of a nucleoside analog RT inhibitor to the in vitro reaction enhanced the overall effectiveness of both drugs, while nonnucleoside analog RT inhibitors exhibited simple additivity Simian immunodeficiency virus
zidovudine triphosphate i.e. AZTTP Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
 i.e. HIV-1
-
Human immunodeficiency virus 2
-
-
-
Simian immunodeficiency virus
-
 i.e. SIVCPZ
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA/DNA hybrid + H2O Cy3-labeled 43-nt RNA strand (5'-GGUCUCUCUGGUUAGACCAGAUCUGAGCCUGGGAGCUCUCUGG-3') annealed to a 53-nt DNA strand (5'-CCCTAGTTAGCCAGAGAGCTCCCAGGCTCAGATCTGGTCTAACCAGAGAGACC-3') Human immunodeficiency virus 1 ?
-
 ?
RNA/DNA hybrid + H2O Cy3-labeled 43-nt RNA strand (5'-GGUCUCUCUGGUUAGACCAGAUCUGAGCCUGGGAGCUCUCUGG-3') annealed to a 53-nt DNA strand (5'-CCCTAGTTAGCCAGAGAGCTCCCAGGCTCAGATCTGGTCTAACCAGAGAGACC-3') Simian immunodeficiency virus ?
-
 ?
RNA/DNA hybrid + H2O Cy3-labeled 43-nt RNA strand (5'-GGUCUCUCUGGUUAGACCAGAUCUGAGCCUGGGAGCUCUCUGG-3') annealed to a 53-nt DNA strand (5'-CCCTAGTTAGCCAGAGAGCTCCCAGGCTCAGATCTGGTCTAACCAGAGAGACC-3') Human immunodeficiency virus 2 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase H
-
 Human immunodeficiency virus 1
RNase H
-
 Simian immunodeficiency virus
RNase H
-
 Human immunodeficiency virus 2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Human immunodeficiency virus 1
37
-
 assay at Simian immunodeficiency virus
37
-
 assay at Human immunodeficiency virus 2

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Human immunodeficiency virus 1
additional information
-
 additional information inhibition kinetics Simian immunodeficiency virus
additional information
-
 additional information inhibition kinetics Human immunodeficiency virus 2

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
-
 Human immunodeficiency virus 1 additional information
additional information
-
-
 Simian immunodeficiency virus additional information
additional information
-
-
 Human immunodeficiency virus 2 additional information