Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant reverse transcriptases in Escherichia coli | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
H539D | site-directed mutagenesis, the mutant enzyme shows an altered cleavage pattern compared to the wild-type enzyme, the mutant is inhibited in performing secondary cleavage directed by the 3' end primer independent of the presence of a hairpin structure or the need to carry out strand displacement | Human immunodeficiency virus 1 |
H539F | site-directed mutagenesis, the mutant enzyme shows an altered cleavage pattern compared to the wild-type enzyme, the mutant is inhibited in performing secondary cleavage directed by the 3' end primer independent of the presence of a hairpin structure or the need to carry out strand displacement | Human immunodeficiency virus 1 |
H539R | site-directed mutagenesis, the mutant enzyme shows an altered cleavage pattern compared to the wild-type enzyme, the mutant is inhibited in performing secondary cleavage directed by the 3' end primer independent of the presence of a hairpin structure or the need to carry out strand displacement | Human immunodeficiency virus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Human immunodeficiency virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | Human immunodeficiency virus 1 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
i.e. HIV-1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
DNA-RNA hybrid + H2O | cleavage pattern to 3' end of wild-type and mutant reverse transcriptase/RNase H, HIV-1 reverse transcriptase employs the DNA 3' end-directed primary/secondary RNase H cleavage mechanism during synthesis and strand transfer | Human immunodeficiency virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is part of the viral reverse transcriptase | Human immunodeficiency virus 1 |
ribonuclease H | - |
Human immunodeficiency virus 1 |
RNase H | - |
Human immunodeficiency virus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human immunodeficiency virus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Human immunodeficiency virus 1 |