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Literature summary for 3.1.13.2 extracted from

  • Julias, J.G.; McWilliams, M.J.; Sarafianos, S.G.; Alvord, W.G.; Arnold, E.; Hughes, S.H.
    Mutation of amino acids in the connection domain of human immunodeficiency virus type 1 reverse transcriptase that contact the template-primer affects RNase H activity (2003), J. Virol., 77, 8548-8554.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.2

Protein Variants

Protein Variants Comment Organism
A360D mutation decreases the virus titer to about 50% of that of the wild-type virus Human immunodeficiency virus 1
E396A mutation decreases the virus titer to about 50% of that of the wild-type virus and has significant effect on the extent of enzyme cleavage Human immunodeficiency virus 1
G359S mutation decreases the virus titer to about 50% of that of the wild-type virus Human immunodeficiency virus 1
H361A mutation decreases the virus titer to about 25% of that of the wild-type virus and affects the frequency of the generation of linear viral DNAs with aberrant ends Human immunodeficiency virus 1
K390A mutation decreases the virus titer less than twofold in comparison of wild-type enzyme Human immunodeficiency virus 1
K395A mutation decreases the virus titer less than twofold in comparison of wild-type enzyme Human immunodeficiency virus 1
T362A mutation decreases the virus titer less than twofold in comparison of wild-type enzyme Human immunodeficiency virus 1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid duplex + H2O Human immunodeficiency virus 1 only cleavage of RNA from a RNA-DNA-duplex oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
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Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
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 HIV-1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid duplex + H2O only cleavage of RNA from a RNA-DNA-duplex Human immunodeficiency virus 1 oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
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 ?