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Literature summary for 3.1.13.2 extracted from

  • Cristofaro, J.V.; Rausch, J.W.; Le Grice, S.F.J.; DeStefano, J.J.
    Mutations in the ribonuclease H active site of HIV-RT reveal a role for this site in stabilizing enzyme-primer-template binding (2002), Biochemistry, 41, 10968-10975.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D549N unaltered polymerase activity, in the absence and at lower concentrations of Mg2+ the mutant enzyme binds the DNA-DNA substrate more tightly than the wild-type enzyme, at high Mg2+ concentrations the binding is identical for the wild-type and mutant enzymes using DNA-DNA template Human Immunodeficiency Virus
E478Q unaltered polymerase activity, in the absence and at lower concentrations of Mg2+ the mutant enzyme binds the DNA-DNA substrate more tightly than the wild-type enzyme, at high Mg2+ concentrations the binding is identical for the wild-type and mutant enzymes using DNA-DNA template Human Immunodeficiency Virus

Inhibitors

Inhibitors Comment Organism Structure
Cl- the wild-type and mutant enzymes bind the substrate considerably less tightly at higher concentrations Human Immunodeficiency Virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in the presence of Mg2+ the RNase H domain clearly stabilizes the association of enzyme with primer-template Human Immunodeficiency Virus

Organism

Organism UniProt Comment Textmining
Human Immunodeficiency Virus
-
 HXB2 strain
-
Human Immunodeficiency Virus HXB2
-
 HXB2 strain
-

Reaction

Reaction Comment Organism Reaction ID
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid The multifunctional enzyme possesses both RNA- and DNA-dependent DNA polymerase activity and an RNase H activity. The activity of enzyme is coordinated by a catalytic triad, E478, D443, D498, of acidic residues that bind divalent cations. Human Immunodeficiency Virus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-DNA hybrid duplex + H2O
-
 Human Immunodeficiency Virus ?
-
 ?
DNA-DNA hybrid duplex + H2O
-
 Human Immunodeficiency Virus HXB2 ?
-
 ?
DNA-RNA hybrid duplex + H2O
-
 Human Immunodeficiency Virus oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?
DNA-RNA hybrid duplex + H2O
-
 Human Immunodeficiency Virus HXB2 oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?