Cloned (Comment) | Organism |
---|---|
pACYC184 derivative expressing RNase II, RNase II lacking cold shock domain-1 or mutant D209N under araBAD promoter. PACYC184 derivative expressing RNase R under araBAD promoter. RNase II and mutant D209N overexpressed from pET plasmid constructs in CH12 DELTArna cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D209N | catalytically inactive, is unable to complement RNase II deletion | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation, ion exchange and hydrophobic interaction chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mRNA + H2O | purified RNase II is unable to directly catalyse A-site cleavage in vitro, RNase II-catalysed degradation of mRNA to the ribosome border is a prerequisite for A-site cleavage. Degrades ribosome-bound mRNA to positions +18 nucleotides downstream of the ribosomal A site | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3'-5'exoribonuclease | - |
Escherichia coli |
RNase II | - |
Escherichia coli |
RNase R | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | instead of A-site cleavage, translational pausing in DELTARNase II cells produces transcripts that are truncated +12 and +28 nucleotides downstream of the A-site codon. Deletion of RNase R has little effect on A-site cleavage. Polynucleotide phosphorylase overexpression restores A-site cleavage activity to DELTARNase II cells | Escherichia coli |
physiological function | plays an important role in RelE-independent A-site cleavage | Escherichia coli |