Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by RNase A-type inhibitor | Homo sapiens | |
poly(A) binding protein | inhibits degradation of poly(A)+-strands | Homo sapiens | |
RNA with a terminal 3'-phosphate group | on the potential substrate molecule | Homo sapiens | |
ss DNA oligonucleotides | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
polysome | - |
Homo sapiens | 5844 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | necessary for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE | Homo sapiens |
37000 | - |
native state gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ss RNA + H2O | Homo sapiens | - |
5'-phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
5300-26500fold, to homogeneity | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythroleukemia cell | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
4°C, 10-ml fractions containing 1 mg trypsin inhibitor carrier protein because RNase II loses activity under this conditions at protein concentrations below 0.1 mg/ml | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
duplex RNA + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | 3'-phosphorylated RNA is not hydrolyzed | Homo sapiens | ? | - |
? | |
mRNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides | - |
? | |
poly(A) + H2O | - |
Homo sapiens | 5'-AMP + oligonucleotide | - |
? | |
ss RNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Homo sapiens |