Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | RecBCD degradation of DNA is inhibited by either cisplatin-damaged or UV-damaged DNA sequences. A DNA-targeted 9-aminoacridinecarboxamide cisplatin analogue is also found to inhibit RecBCD activity. The three different DNA damaging agents, UV light, cisplatin and 9AmAcPtCl2, are examined, all strongly inhibit the activity of RecBCD | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | RecBCD degradation of DNA is inhibited by either cisplatin-damaged or UV-damaged DNA sequences | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P08394 AND P07648 AND P04993 | RecBCD enzyme subunits RecB, RecC, and RecD, i.e. beta-, gamma-, and alpha-subunits | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RecBCD degradation of DNA is inhibited by either cisplatin-damaged or UV-damaged DNA sequences | Escherichia coli | ? | - |
? | |
additional information | assay substrate is PvuII-cut plasmid DNA. when the RecBCD enzyme encounters a Chi site (5'-GCTGGTGG), translocation of the RecBCD pauses and the nuclease digestion of the 30-tail stops | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exonuclease V | - |
Escherichia coli |
RecBCD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | RecBCD (exonuclease V) plays a critical role in recombinational DNA repair. Escherichia coli RecBCD is involved in recombinational repair of double-strand breaks that are caused by defective DNA replication and other DNA damaging agents. Unrepaired DNA damage caused by cisplatin or UV light can lead to defective DNA replication and the production of double-strand breaks. Presence of adducts on the DNA double-helix can have major consequences for the efficient functioning of DNA repair enzymes. Escherichia coli RecBCD (exonuclease V) is involved in recombinational repair of double-strand breaks that are caused by defective DNA replication, DNA damaging agents and other factors. The holoenzyme possesses a bipolar helicase activity which helps unwind DNA from both 3'- and 5'-directions and is coupled with a potent exonuclease activity that is also capable of digesting DNA from both 3'- and 5'-ends | Escherichia coli |