Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.11.5 extracted from

  • Leung, W.Y.; Chung, L.H.; Kava, H.W.; Murray, V.
    RecBCD (exonuclease V) is inhibited by DNA adducts produced by cisplatin and ultraviolet light (2018), Biochem. Biophys. Res. Commun., 495, 666-671 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information RecBCD degradation of DNA is inhibited by either cisplatin-damaged or UV-damaged DNA sequences. A DNA-targeted 9-aminoacridinecarboxamide cisplatin analogue is also found to inhibit RecBCD activity. The three different DNA damaging agents, UV light, cisplatin and 9AmAcPtCl2, are examined, all strongly inhibit the activity of RecBCD Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli RecBCD degradation of DNA is inhibited by either cisplatin-damaged or UV-damaged DNA sequences ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P08394 AND P07648 AND P04993 RecBCD enzyme subunits RecB, RecC, and RecD, i.e. beta-, gamma-, and alpha-subunits
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information RecBCD degradation of DNA is inhibited by either cisplatin-damaged or UV-damaged DNA sequences Escherichia coli ?
-
?
additional information assay substrate is PvuII-cut plasmid DNA. when the RecBCD enzyme encounters a Chi site (5'-GCTGGTGG), translocation of the RecBCD pauses and the nuclease digestion of the 30-tail stops Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
exonuclease V
-
Escherichia coli
RecBCD
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli

General Information

General Information Comment Organism
physiological function RecBCD (exonuclease V) plays a critical role in recombinational DNA repair. Escherichia coli RecBCD is involved in recombinational repair of double-strand breaks that are caused by defective DNA replication and other DNA damaging agents. Unrepaired DNA damage caused by cisplatin or UV light can lead to defective DNA replication and the production of double-strand breaks. Presence of adducts on the DNA double-helix can have major consequences for the efficient functioning of DNA repair enzymes. Escherichia coli RecBCD (exonuclease V) is involved in recombinational repair of double-strand breaks that are caused by defective DNA replication, DNA damaging agents and other factors. The holoenzyme possesses a bipolar helicase activity which helps unwind DNA from both 3'- and 5'-directions and is coupled with a potent exonuclease activity that is also capable of digesting DNA from both 3'- and 5'-ends Escherichia coli