Any feedback?
Literature summary for 3.1.11.5 extracted from
- Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation (2008), Anal. Biochem., 377, 156-161.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | RecD protein expression level is decreased at lower cultivation temperature, which greatly improves the productivity of cell-free protein synthesis from linear DNA templates | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
BL21 strain and BL21-RecD-SBP strain | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | BL21 and BL21-RecD-SBP strains share similar linear DNA degradation activities | Escherichia coli | ? | - |
? | |
| additional information | RecD protein expression level is decreased at lower cultivation temperature, which greatly improves the productivity of cell-free protein synthesis from linear DNA templates | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exonuclease V | - |
Escherichia coli |
| RecD | - |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
by altering the cultivation temperature (37°C) of the cells to a moderately lower range (20-34°C), dramatically reduces the linear DNA degradation activity of RecD | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
