Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.11.5 extracted from

  • Eggleston, A.K.; Kowalczykowski, S.C.
    Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity (1993), J. Mol. Biol., 231, 605-620.
    View publication on PubMed
Search for more literature for 3.1.11.5

Application

Application Comment Organism
analysis no recombination in recB2109CD mutants Escherichia coli
analysis possible role in genetic recombination Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal concentration around 3-12 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Escherichia coli
-
 ADP + phosphate
-
 ?
double-stranded DNA + H2O Escherichia coli responsible for several steps in genetic recombination process single-stranded DNA fragments
-
 ?
double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
 ?
double-stranded DNA + H2O Escherichia coli ATP-dependent helicase single-stranded DNA fragments
-
 ?
single stranded DNA + H2O Escherichia coli ATP-dependent exonuclease 5'-phosphomonoester oligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 recB2109CD mutant
-

Purification (Commentary)

Purification (Comment) Organism
of the abc-modified recBCD protein Escherichia coli
of the mutant recB2109CD mutant Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
ATP + H2O reduced activity in recB2109CD mutant Escherichia coli ADP + phosphate
-
 ?
double-stranded DNA no recombination in recB2109CD mutants and abc-modified recBCD mutants Escherichia coli intermediates with single stranded regions
-
 ?
double-stranded DNA recB2109CD mutant has no chi nicking activity Escherichia coli intermediates with single stranded regions
-
 ?
double-stranded DNA + H2O reduced activity in recB2109CD mutant Escherichia coli single-stranded DNA fragments
-
 ?
double-stranded DNA + H2O responsible for several steps in genetic recombination process Escherichia coli single-stranded DNA fragments
-
 ?
double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
 ?
double-stranded DNA + H2O ATP-dependent helicase Escherichia coli single-stranded DNA fragments
-
 ?
single stranded DNA + H2O reduced activity in recB2109CD mutant Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
single stranded DNA + H2O ATP dependence varies Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
single stranded DNA + H2O ATP-dependent exonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?