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Literature summary for 3.1.11.5 extracted from

  • Boehmer, P.E.; Emmerson, P.T.
    Escherichia coli RecBCD enzyme: inducible overproduction and reconstitution of the ATP-dependent deoxyribonuclease from purified subunits (1991), Gene, 102, 1-6.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
fusion of the recBCD genes and expression in Escherichia coli Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli
130000
-
1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli
140000
-
1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant proteins B, C and D Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O no exonuclease activity in recD null mutants Escherichia coli single-stranded DNA fragments
-
?
double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
?

Subunits

Subunits Comment Organism
trimer 1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli