Any feedback?
Literature summary for 3.1.11.5 extracted from
- Biochemical and physical characterization of exonuclease V from Escherichia coli. Comparison of the catalytic activities of the RecBC and RecBCD enzymes (1990), J. Biol. Chem., 265, 3447-3454.
General Stability
| General Stability | Organism |
|---|---|
| RecBC enzyme more instable than the recBCD enzyme | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
recD protein, sedimentation, SDS-PAGE | Escherichia coli |
| 270000 | - |
gel filtration, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? |  |
| double-stranded DNA + H2O | Escherichia coli | ATP-dependent exonuclease | single-stranded DNA fragments | - |
? | |
| double-stranded DNA + H2O | Escherichia coli | ATP-dependent helicase | single-stranded DNA fragments | - |
? | |
| single stranded DNA + H2O | Escherichia coli | ATP-dependent exonuclease | 5'-phosphomonoester oligonucleotides | - |
? | |
| single stranded DNA + H2O | Escherichia coli | ATP-dependent endonuclease | 5'-phosphomonoester oligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme RecBC, 1 year, 80% loss of activity | Escherichia coli |
| -20°C, purified enzyme RecBCD, 1 year, 10%-20% loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
| ATP + H2O | ATPase activity in recBC enzyme | Escherichia coli | ADP + phosphate | - |
? | |
| double-stranded DNA | unwinding activity in the recBC enzyme | Escherichia coli | intermediates with single stranded regions | - |
? | |
| double-stranded DNA + H2O | no exonuclease activity in recD null mutants | Escherichia coli | single-stranded DNA fragments | - |
? | |
| double-stranded DNA + H2O | ATP-dependent exonuclease | Escherichia coli | single-stranded DNA fragments | - |
? | |
| double-stranded DNA + H2O | ATP-dependent helicase | Escherichia coli | single-stranded DNA fragments | - |
? | |
| single stranded DNA + H2O | no exonuclease activity in recD null mutants | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
| single stranded DNA + H2O | endonuclease activity is ATP dependent in recBC enzyme | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
| single stranded DNA + H2O | also endonuclease activity on circular DNA | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
| single stranded DNA + H2O | ATP dependence varies | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
| single stranded DNA + H2O | ATP-dependent exonuclease | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
| single stranded DNA + H2O | ATP-dependent endonuclease | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
