Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 0.2 mM and higher concentrations inhibits phosphodiester hydrolysis; neither the rate nor the extent of hydrolysis of single-stranded DNA nor ATP is affected | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
ATP | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42500 | - |
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor | Bacillus subtilis |
52500 | - |
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor | Bacillus subtilis |
62000 | - |
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor | Bacillus subtilis |
70000 | - |
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor | Bacillus subtilis |
81000 | - |
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor | Bacillus subtilis |
308000 | - |
gel filtration, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Bacillus subtilis | - |
ADP + phosphate | - |
? | |
double-stranded DNA + H2O | Bacillus subtilis | - |
single-stranded DNA fragments | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Bacillus subtilis | ADP + phosphate | - |
? | |
double-stranded DNA + H2O | - |
Bacillus subtilis | single-stranded DNA fragments | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor | Bacillus subtilis |