General Stability | Organism |
---|---|
DNA stabilizes against thermal denaturation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | inhibitory at 40 mM and higher concentrations | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Escherichia coli | ATP-dependent exonuclease | single-stranded DNA fragments | - |
? | |
single stranded DNA + H2O | Escherichia coli | ATP-dependent endonuclease | 5'-phosphomonoester oligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
rorA mutant | - |
Purification (Comment) | Organism |
---|---|
to homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | slower degradation of UV-irradiated DNA | Escherichia coli | single-stranded DNA fragments | - |
? | |
double-stranded DNA + H2O | ATP-dependent exonuclease | Escherichia coli | single-stranded DNA fragments | - |
? | |
single stranded DNA + H2O | also endonuclease activity on circular DNA | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
single stranded DNA + H2O | ATP-dependent endonuclease | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
faster inactivation in presence of ATP | Escherichia coli |
42 | - |
complete stable in presence of DNA for 40 min | Escherichia coli |