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Literature summary for 3.1.11.2 extracted from

  • Shiraishi, M.; Ishino, S.; Heffernan, M.; Cann, I.; Ishino, Y.
    The mesophilic archaeon Methanosarcina acetivorans counteracts uracil in DNA with multiple enzymes EndoQ, ExoIII, and UDG (2018), Sci. Rep., 8, 15791 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina acetivorans

Protein Variants

Protein Variants Comment Organism
E39A inactive mutant enzyme ExoIII Methanosarcina acetivorans

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8TP42
-
-
Methanosarcina acetivorans ATCC 35395 Q8TP42
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme digests blunt-ended dsDNA successively. ssDNA remains almost completely intact. 3'-recessed dsDNA is digested in the same manner as blunt-ended dsDNA. 3'-protruding ended dsDNA shows resistance to degradation. The enzyme exhibits dsDNA-specific exonuclease activity from the 3' to 5' direction. A nick site in dsDNA and dsDNA with 3'-phosphate termini are also susceptible to digestion. The enzyme can exhibit exonuclease activity from a nick and possesses 3'-phosphomonoesterase activity. The limited length of the product is observed to be 11 nt. the enzyme may require at least 11-nt DNA for binding Methanosarcina acetivorans ?
-
 ?
DNA + H2O the enzyme digests blunt-ended dsDNA successively. ssDNA remains almost completely intact. 3'-recessed dsDNA is digested in the same manner as blunt-ended dsDNA. 3'-protruding ended dsDNA shows resistance to degradation. The enzyme exhibits dsDNA-specific exonuclease activity from the 3' to 5' direction. A nick site in dsDNA and dsDNA with 3'-phosphate termini are also susceptible to digestion. The enzyme can exhibit exonuclease activity from a nick and possesses 3'-phosphomonoesterase activity. The limited length of the product is observed to be 11 nt. the enzyme may require at least 11-nt DNA for binding Methanosarcina acetivorans ATCC 35395 ?
-
 ?

Synonyms

Synonyms Comment Organism
MacExoIII
-
 Methanosarcina acetivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina acetivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanosarcina acetivorans

General Information

General Information Comment Organism
physiological function the enzyme is involved in uracil rapair pathway Methanosarcina acetivorans