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Literature summary for 3.1.11.2 extracted from
- A simple turn-on fluorescent biosensor for sensitive detection of exonuclease III activity through photoinduced electron transfer and self-hybridization of a DNA probe (2018), Anal. Methods, 10, 2257-2262 .
No PubMed abstract available
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9NSU2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FAM-labeled dsDNA + H2O | Exo III catalyzes the stepwise removal of mononucleotides from the 3'-hydroxyl termini of double-stranded DNA (dsDNA). Development of a fluorescence detection method of Exo III activity, overview. Due to photoinduced electron transfer (PET) between FAM and guanine (G), the fluorescence of the FAMlabeled dsDNA probe is completely quenched. Upon the addition of Exo III, the dsDNA will be digested, and FAM emits very strong fluorescence. Thus, Exo III activity can be facilely measured with a simple fluorescence reader with a detection range from 5 x 10-4 U/ml to 5 U/ml with a detection limit of 3 x 10-4 U/ml. FAM is labeled on the 3' end. Optimization of experimental conditions, overview. HIgh selectivity of the assay for detection of Exo III activity compared to other nucleases | Homo sapiens | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo III | - |
Homo sapiens |
| exonuclease III | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
20 min, inactivation | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | exonuclease III (Exo III) plays crucial roles in maintaining the genome stability | Homo sapiens |
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Release 2023.1 (January 2023)
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