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Literature summary for 3.1.11.2 extracted from

  • Yang, C.J.; Cui, L.; Huang, J.; Yan, L.; Lin, X.; Wang, C.; Zhang, W.Y.; Kang, H.
    Linear molecular beacons for highly sensitive bioanalysis based on cyclic Exo III enzymatic amplification (2011), Biosens. Bioelectron., 27, 119-124.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli Exo III catalyzes stepwise removal of mononucleotides from blunt or recessed 3'-hydroxyl termini of duplex DNA, while it is not active on single-stranded DNA or 3'-protruding termini of double-stranded DNA. Different from restriction enzymes, Exo III does not require any specific enzymatic recognition sequence to function ?
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Exo III catalyzes stepwise removal of mononucleotides from blunt or recessed 3'-hydroxyl termini of duplex DNA, while it is not active on single-stranded DNA or 3'-protruding termini of double-stranded DNA. Different from restriction enzymes, Exo III does not require any specific enzymatic recognition sequence to function Escherichia coli ?
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?
additional information optimization of sequence analysis system using the enzyme with linear molecular beacons, i.e. linear oligonucleotide probes with a fluorophore and quencher attached to 3' terminal and penultimate nucleotides, respectively, in Exo III amplification assays to improve hybridization kinetics and reduce background noises, overview Escherichia coli ?
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?

Synonyms

Synonyms Comment Organism
exo III
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Escherichia coli
exonuclease III
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli