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Literature summary for 3.1.11.2 extracted from
- A label-free assay of exonuclease activity using a pyrosequencing technique (2010), Anal. Biochem., 405, 11-18.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | development of a label-free assay for the in vitro determination of double-stranded DNA-specific 3'-5' exonuclease, 35dsExo, activity possessing the high sensitivity and high-throughput potential of an established pyrosequencing protocol, overview | Escherichia coli | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3'-5' exonuclease | - |
Escherichia coli |
| exo III | - |
Escherichia coli |
| exonuclease III | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 30 | assay at 30°C or at room temperature | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 37 | low activity at 37°C | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
10 min, inactivation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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