Any feedback?
Literature summary for 3.1.11.2 extracted from
- Mechanism of action of Escherichia coli exonuclease III (1989), Biochemistry, 28, 3280-3287.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | sharp optimum requirement at 1 mM for exonucleolytic and endonucleolytic activity | Escherichia coli |  |
| Mg2+ | broad optimum, requirement for exonucleolytic and endonucleolytic activity | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | recognizes apurinic sites, reduced apurinic sites, urea and O-alkylhydroxylamine residues of PM2 DNA | Escherichia coli | additional information | - |
? | |
| double-stranded DNA + H2O | hydrolyzes the phosphodiester bond 5'- to O-alkylhydroxylamine residue | Escherichia coli | additional information | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
in presence of Mg2+, exonuclease III exists in two temperature-dependent conformations. The transition temperature for the conformation change occurs at 25°C | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
