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Literature summary for 3.1.11.1 extracted from

  • Tori, K.; Ishino, S.; Kiyonari, S.; Tahara, S.; Ishino, Y.
    A novel single-strand specific 3'-5' exonuclease found in the hyperthermophilic archaeon, Pyrococcus furiosus (2013), PLoS One, 8, e58497.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent upon a divalent cation, and Mg2+ is the most preferable Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25596
-
3 * 25596, calculated from sequence Pyrococcus furiosus
73400
-
gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8TZE9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no endonuclease activity with either ssDNA or dsDNA Pyrococcus furiosus ?
-
?
single-stranded oligodeoxyribonucleotide + H2O excises the DNA at every two nucleotides from the 3' to 5' direction. The enzyme needs to grasp a 4–6 nt-long DNA strand to express its nucleolytic activity. The homo dT 30 nt-long homooligomer is the most preferable substrate for PfuExo I Pyrococcus furiosus single-stranded oligodeoxyribonucleotide + nucleoside 5'-monophosphate
-
?

Subunits

Subunits Comment Organism
trimer 3 * 25596, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
PF2046
-
Pyrococcus furiosus
PfuExo I
-
Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
assay at Pyrococcus furiosus