Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent upon a divalent cation, and Mg2+ is the most preferable | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25596 | - |
3 * 25596, calculated from sequence | Pyrococcus furiosus |
73400 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8TZE9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no endonuclease activity with either ssDNA or dsDNA | Pyrococcus furiosus | ? | - |
? | |
single-stranded oligodeoxyribonucleotide + H2O | excises the DNA at every two nucleotides from the 3' to 5' direction. The enzyme needs to grasp a 46 nt-long DNA strand to express its nucleolytic activity. The homo dT 30 nt-long homooligomer is the most preferable substrate for PfuExo I | Pyrococcus furiosus | single-stranded oligodeoxyribonucleotide + nucleoside 5'-monophosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 25596, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PF2046 | - |
Pyrococcus furiosus |
PfuExo I | - |
Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Pyrococcus furiosus |