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Literature summary for 3.1.11.1 extracted from

  • Eid, W.; Steger, M.; El-Shemerly, M.; Ferretti, L.P.; Pena-Diaz, J.; Koenig, C.; Valtorta, E.; Sartori, A.A.; Ferrari, S.
    DNA end resection by CtIP and exonuclease 1 prevents genomic instability (2010), EMBO Rep., 11, 962-968.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.1

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of GFP-tagged EXO1 in U2OS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information various DNA damage phenotypes in response to camptothecin occur after siRNA-mediated downregulation of CtIP and EXO1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
camptothecin on exposure to camptothecin, depletion of EXO1 in CtIP-deficient cells increases the frequency of DNA-PK-dependent radial chromosome formation Homo sapiens
endonuclease CtIP inhibitory effect of CtIP on EXO1 activity using either a radiolabelled DNA oligonucleotide substrate or a linearized plasmid both containing 3'-overhangs Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus EXO1 is bound to CtIP Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a radiolabelled DNA oligonucleotide substrate or a linearized plasmid both containing 3'-overhangs are the preferred substrate for EXO1 in vitro. Pre-incubation of CtIP with either blunt-ended or 5'-overhang substrates facilitated processing by EXO1, which does not occur when the proteins are added in the reverse order Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Exo1
-
 Homo sapiens
exonuclease 1
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction on exposure to camptothecin, depletion of EXO1 in CtIP-deficient cells increases the frequency of DNA-PK-dependent radial chromosome formation Homo sapiens
additional information two-step model of DNA end resection Homo sapiens
physiological function end resection of DNA, which is essential for the repair of DNA double-strand breaks by homologous recombination, relies first on the partnership between MRE11-RAD50-NBS1 and CtIP, followed by a processive step involving helicases and exonucleases such as exonuclease 1. Localization of EXO1 to double-strand breaks depends on both CtIP and MRE11-RAD50-NBS1. CtIP and EXO1 cooperate to prevent non-homologous end-joining Homo sapiens