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Literature summary for 3.1.11.1 extracted from

  • Ray, R.K.; Reuben, R.; Molineux, I.; Gefter, M.
    The purification of exonuclease I from Escherichia coli by affinity chromatography (1974), J. Biol. Chem., 249, 5379-5381.
    View publication on PubMed
Search for more literature for 3.1.11.1

Inhibitors

Inhibitors Comment Organism Structure
EDTA activity 88% reduced in the presence of Mg2+ Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 2 * 70000, native PAGE Escherichia coli
140000
-
 native PAGE and SDS-PAGE, 85% of activity migrated as dimer, 15% as monomer Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
single-stranded polydeoxyribonucleotide + H2O Escherichia coli
-
 single-stranded oligodeoxynucleotide + nucleoside 5'-monophosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneitiy by affinity chromatography Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Escherichia coli
-

Storage Stability

Storage Stability Organism
on ice, 0.02 M Tris-HCl pH 7.4, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10% glycerol, 30% polyethylene glycol, 2 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded polydeoxyribonucleotide + H2O
-
 Escherichia coli single-stranded oligodeoxynucleotide + nucleoside 5'-monophosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 70000, native PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Escherichia coli