BRENDA - Enzyme Database
show all sequences of 3.1.1.B11

Functional expression of a thermophilic glucuronyl esterase from Sporotrichum thermophile identification of the nucleophilic serine

Topakas, E.; Moukouli, M.; Dimarogona, M.; Vafiadi, C.; Christakopoulos, P.; Appl. Microbiol. Biotechnol. 87, 1765-1772 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Pichia pastoris
Thermothelomyces thermophilus
Engineering
Amino acid exchange
Commentary
Organism
S213A
complete loss of activity
Thermothelomyces thermophilus
Inhibitors
Inhibitors
Commentary
Organism
Structure
PMSF
10 mM, 10 min, 60% loss of activity
Thermothelomyces thermophilus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Thermothelomyces thermophilus
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermothelomyces thermophilus
G2QJR6
-
-
Thermothelomyces thermophilus DSM 1799
G2QJR6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
741731
Thermothelomyces thermophilus
methanol + 4-O-methyl-D-glucopyranuronate
-
-
-
?
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
741731
Thermothelomyces thermophilus DSM 1799
methanol + 4-O-methyl-D-glucopyranuronate
-
-
-
?
additional information
enzyme is active on substrates containing glucuronic acid methyl ester
741731
Thermothelomyces thermophilus
?
-
-
-
-
additional information
enzyme is active on substrates containing glucuronic acid methyl ester
741731
Thermothelomyces thermophilus DSM 1799
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 42881, calulated, x * 43000, recombinant protein with His-tag
Thermothelomyces thermophilus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Thermothelomyces thermophilus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
60
70
more than 70% of maximum activity
Thermothelomyces thermophilus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to
Thermothelomyces thermophilus
55
-
pH 7.0, 24 h, 53% loss of activity
Thermothelomyces thermophilus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Thermothelomyces thermophilus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
10
-
Thermothelomyces thermophilus
7
-
55°C, 24 h, 53% loss of activity
Thermothelomyces thermophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Pichia pastoris
Thermothelomyces thermophilus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
S213A
complete loss of activity
Thermothelomyces thermophilus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
PMSF
10 mM, 10 min, 60% loss of activity
Thermothelomyces thermophilus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Thermothelomyces thermophilus
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
741731
Thermothelomyces thermophilus
methanol + 4-O-methyl-D-glucopyranuronate
-
-
-
?
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
741731
Thermothelomyces thermophilus DSM 1799
methanol + 4-O-methyl-D-glucopyranuronate
-
-
-
?
additional information
enzyme is active on substrates containing glucuronic acid methyl ester
741731
Thermothelomyces thermophilus
?
-
-
-
-
additional information
enzyme is active on substrates containing glucuronic acid methyl ester
741731
Thermothelomyces thermophilus DSM 1799
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42881, calulated, x * 43000, recombinant protein with His-tag
Thermothelomyces thermophilus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Thermothelomyces thermophilus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
60
70
more than 70% of maximum activity
Thermothelomyces thermophilus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to
Thermothelomyces thermophilus
55
-
pH 7.0, 24 h, 53% loss of activity
Thermothelomyces thermophilus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Thermothelomyces thermophilus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
10
-
Thermothelomyces thermophilus
7
-
55°C, 24 h, 53% loss of activity
Thermothelomyces thermophilus
General Information
General Information
Commentary
Organism
physiological function
the consensus sequence G-C-S-R-X-G features the characteristic serine residue involved in the generally conserved catalytic mechanism of the esterase family
Thermothelomyces thermophilus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the consensus sequence G-C-S-R-X-G features the characteristic serine residue involved in the generally conserved catalytic mechanism of the esterase family
Thermothelomyces thermophilus
Other publictions for EC 3.1.1.B11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749697
Huynh
A novel glucuronoyl esterase ...
Aspergillus fumigatus, Aspergillus fumigatus ATCC MYA-4609
Appl. Microbiol. Biotechnol.
102
2191-2201
2018
-
1
1
-
-
-
11
4
-
1
1
-
-
6
-
-
1
-
-
-
-
-
8
-
1
1
3
4
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
11
-
4
-
1
1
-
-
-
-
1
-
-
-
-
8
-
1
1
3
4
1
-
3
-
-
-
-
-
4
4
749713
Lin
Classification of fungal gluc ...
Gelatoporia subvermispora, Pleurotus eryngii
Appl. Microbiol. Biotechnol.
102
9635-9645
2018
-
-
2
-
-
-
15
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
15
-
2
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
2
-
-
-
2
-
-
-
-
2
2
750137
Mosbech
The natural catalytic functio ...
Cerrena unicolor
Biotechnol. Biofuels
11
71
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751638
Dilokpimol
Fungal glucuronoyl esterases ...
Arthrinium arundinis, Ascobolus immersus, Botryosphaeria dothidea, Dichomitus squalens, Dichomitus squalens LYAD-421, Hypholoma sublateritium, Hypholoma sublateritium FD-334 SS-4, Leptosphaeria maculans, Parastagonospora nodorum, Penicillium rubens, Penicillium rubens ATCC 28089, Phanerochaete carnosa, Phanerochaete carnosa HHB-10118-sp, Piromyces sp. E2, Podospora anserina, Podospora anserina FGSC 10383, Schizophyllum commune, Schizophyllum commune H4-8, Serendipita indica, Serendipita indica DSM 11827, Stereum hirsutum, Thermothelomyces thermophilus, Thermothelomyces thermophilus ATCC 42464, Trichoderma reesei, Trichoderma reesei QM6a
N. Biotechnol.
40
282-287
2018
-
-
-
-
-
-
-
-
-
-
18
-
-
36
-
12
-
-
-
-
17
-
27
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
13
-
-
-
17
-
27
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
749690
Hüttner
Characterisation of three fun ...
Acremonium alcalophilum, Phanerochaete chrysosporium, Phanerochaete chrysosporium RP-78, Wolfiporia cocos, Wolfiporia cocos MD-104
Appl. Microbiol. Biotechnol.
101
5301-5311
2017
-
3
3
-
-
-
-
3
-
-
-
-
-
12
-
-
3
-
-
-
-
-
15
-
-
-
8
3
-
-
15
-
-
-
-
-
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
-
-
-
-
15
-
-
-
8
3
-
-
15
-
-
-
-
-
-
-
749564
Franova
beta-Glucuronidase-coupled as ...
Ruminococcus flavefaciens, Schizophyllum commune, Schizophyllum commune H4-8, Trichoderma reesei, Trichoderma reesei QM6a
Anal. Biochem.
510
114-119
2016
-
3
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749662
Biely
Microbial glucuronoyl esteras ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium RP-78, Podospora anserina, Podospora anserina S, Ruminococcus flavefaciens, Schizophyllum commune, Schizophyllum commune H4-8, Teredinibacter turnerae, Teredinibacter turnerae ATCC 39867, Trichoderma reesei, Trichoderma reesei QM6a
Appl. Environ. Microbiol.
82
7014-7018
2016
-
6
-
-
-
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
12
-
-
-
750544
Arnling Baath
-
A glucuronoyl esterase from A ...
Acremonium alcalophilum
FEBS Lett.
590
2611-2618
2016
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
751153
dErrico
Improved biomass degradation ...
Cerrena unicolor, Schizophyllum commune, Schizophyllum commune H4-8, Trichoderma reesei
J. Biotechnol.
219
117-123
2016
-
3
3
-
-
-
-
2
-
-
1
-
-
8
-
1
3
-
-
-
-
-
3
1
3
-
1
2
3
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
3
-
-
-
-
3
1
3
-
1
2
3
-
1
-
-
-
-
-
2
2
751237
Huynh
Functional expression and cha ...
Neurospora crassa, Neurospora crassa FGSC 987
J. Gen. Appl. Microbiol.
62
217-224
2016
1
-
1
-
-
-
14
-
1
4
1
-
-
5
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
14
-
-
1
4
1
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
1
1
-
-
-
751621
Sunner
Glucuronoyl esterase screenin ...
Cerrena unicolor, Phanerochaete chrysosporium, Phanerochaete chrysosporium RP-78, Podospora anserina, Podospora anserina DSM 980, Schizophyllum commune, Schizophyllum commune H4-8, Thermothelomyces thermophilus, Thermothelomyces thermophilus DSM 1799, Trichoderma reesei, Trichoderma reesei QM6a
Molecules
20
17807-17817
2015
-
12
-
-
-
-
-
-
-
-
-
-
-
26
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751818
Gandla
Expression of a fungal glucur ...
Phanerochaete carnosa, Phanerochaete carnosa HHB-10118-sp
Phytochemistry
112
210-220
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741739
Katsimpouras
Enzymatic synthesis of model ...
Podospora anserina, Podospora anserina DSM 980, Thermothelomyces thermophilus, Thermothelomyces thermophilus DSM 1799
Appl. Microbiol. Biotechnol.
98
5507-5516
2014
-
-
2
2
-
-
-
5
1
-
-
-
-
14
-
-
-
-
-
-
-
-
16
1
-
-
-
5
-
-
-
-
-
1
-
-
-
2
-
2
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
16
1
-
-
-
5
-
-
-
1
-
-
-
-
5
5
741509
Charavgi
The structure of a novel gluc ...
Thermothelomyces thermophilus, Thermothelomyces thermophilus DSM 1799
Acta Crystallogr. Sect. D
69
63-73
2013
-
-
-
1
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741731
Topakas
Functional expression of a th ...
Thermothelomyces thermophilus, Thermothelomyces thermophilus DSM 1799
Appl. Microbiol. Biotechnol.
87
1765-1772
2010
-
-
1
-
1
-
1
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
4
1
1
1
2
-
1
-
2
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
1
1
1
2
-
1
-
2
-
-
1
1
-
-
-
742506
Li
Identification of genes encod ...
Trichoderma reesei, Trichoderma reesei QM6a
FEBS Lett.
581
4029-4035
2007
-
-
1
-
-
-
2
1
1
-
1
-
-
11
-
-
-
-
-
-
-
-
6
1
-
1
-
1
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
2
-
1
1
-
1
-
-
-
-
-
-
-
-
-
6
1
-
1
-
1
1
-
-
1
1
1
1
1
1
1