BRENDA - Enzyme Database
show all sequences of 3.1.1.B10

Clostridium thermocellum releases coumaric acid during degradation of untreated grasses by the action of an unknown enzyme

Herring, C.D.; Thorne, P.G.; Lynd, L.R.; Appl. Microbiol. Biotechnol. 100, 2907-2915 (2016)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hungateiclostridium thermocellum
-
-
-
Hungateiclostridium thermocellum ATCC 27405
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bagasse + H2O
-
735517
Hungateiclostridium thermocellum
coumaric acid + ?
-
-
-
?
bagasse + H2O
-
735517
Hungateiclostridium thermocellum ATCC 27405
coumaric acid + ?
-
-
-
?
switchgrass + H2O
-
735517
Hungateiclostridium thermocellum
coumaric acid + ?
-
-
-
?
switchgrass + H2O
-
735517
Hungateiclostridium thermocellum ATCC 27405
coumaric acid + ?
-
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bagasse + H2O
-
735517
Hungateiclostridium thermocellum
coumaric acid + ?
-
-
-
?
bagasse + H2O
-
735517
Hungateiclostridium thermocellum ATCC 27405
coumaric acid + ?
-
-
-
?
switchgrass + H2O
-
735517
Hungateiclostridium thermocellum
coumaric acid + ?
-
-
-
?
switchgrass + H2O
-
735517
Hungateiclostridium thermocellum ATCC 27405
coumaric acid + ?
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
cellulase preparations from a mutant strain lacking the scaffoldin cipA still show activity, though diminished. Deletion of all three proteins in Clostridium thermocellum with ferulic acid esterase domains, either singly or in combination, does not eliminate the activity
Hungateiclostridium thermocellum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
cellulase preparations from a mutant strain lacking the scaffoldin cipA still show activity, though diminished. Deletion of all three proteins in Clostridium thermocellum with ferulic acid esterase domains, either singly or in combination, does not eliminate the activity
Hungateiclostridium thermocellum
Other publictions for EC 3.1.1.B10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750588
Siebert
Enzymatic mitigation of 5-O-c ...
Rhizoctonia solani
Food Chem.
258
124-128
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
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-
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-
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1
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-
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-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745896
Nieter
A p-coumaroyl esterase from R ...
Rhizoctonia solani
New Biotechnol.
37
153-161
2017
-
-
1
-
-
-
-
3
1
-
-
-
-
7
-
-
1
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1
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8
-
1
1
2
3
1
1
-
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-
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1
-
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-
-
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3
1
-
-
-
-
-
-
1
-
1
-
-
8
-
1
1
2
3
1
1
-
-
-
-
-
-
3
3
735517
Herring
Clostridium thermocellum relea ...
Hungateiclostridium thermocellum, Hungateiclostridium thermocellum ATCC 27405
Appl. Microbiol. Biotechnol.
100
2907-2915
2016
-
-
-
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-
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9
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4
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4
-
-
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-
-
-
-
-
-
-
1
1
-
-
-
735472
Borneman
Isolation and characterization ...
Neocallimastix sp. MC-2
Appl. Environ. Microbiol.
57
2337-2344
1991
-
-
-
-
-
-
7
1
1
-
2
-
1
3
-
-
1
-
-
-
3
1
5
1
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
7
-
1
1
-
2
-
1
-
-
1
-
-
3
1
5
1
1
-
1
-
1
-
1
1
-
1
1
-
-
-
735427
Borneman
Assay for trans-p-coumaroyl es ...
Neocallimastix sp. MC-2
Anal. Biochem.
190
129-133
1990
-
1
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
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-
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-
735523
Borneman
-
Feruloyl and p-coumaroyl ester ...
Neocallimastix sp. MC-2
Appl. Microbiol. Biotechnol.
33
345-351
1990
-
-
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-
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1
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1
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2
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1
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2
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1
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1
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