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Literature summary for 3.1.1.B10 extracted from

  • Borneman, W.S.; Ljungdahl, L.G.; Hartley, R.D.; Akin, D.E.
    Isolation and characterization of p-coumaroyl esterase from the anaerobic fungus Neocallimastix strain MC-2 (1991), Appl. Environ. Microbiol., 57, 2337-2344.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.B10

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 23% inhibition Neocallimastix sp. MC-2
Cu2+ 5 mM, 16% inhibition Neocallimastix sp. MC-2
dithiothreitol 10 mM, 26% inhibition Neocallimastix sp. MC-2
Fe2+ 1 mM, 19% inhibition Neocallimastix sp. MC-2
Hg2+ 1 mM, 58% inhibition Neocallimastix sp. MC-2
NaCl 150 mM, 15% inhibition Neocallimastix sp. MC-2
SDS 0.1%, 85% inhibition Neocallimastix sp. MC-2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0194
-
 [5-O-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose pH 7.2, 40°C Neocallimastix sp. MC-2

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Neocallimastix sp. MC-2
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5800
-
 2 * 5800, SDS-PAGE Neocallimastix sp. MC-2
11000
-
 PAGE Neocallimastix sp. MC-2

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol 15% V/V, 12% increase in activity Neocallimastix sp. MC-2

Organism

Organism UniProt Comment Textmining
Neocallimastix sp. MC-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification from culture medium Neocallimastix sp. MC-2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.34
-
 O-beta-D-xylopyranosyl-(1->4)-O-[5-O-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl(1->3)]-O-beta-D-xylopyranosyl-(1->4)-D-xylopyranose, pH 7.0, 40°C Neocallimastix sp. MC-2
4.91
-
 substrate [5-O-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose, pH 7.0, 40°C Neocallimastix sp. MC-2
6.01
-
 substrate beta-D-xylopyranosyl-(1->2)-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose, pH 7.0, 40°C Neocallimastix sp. MC-2

Storage Stability

Storage Stability Organism
-80°C, 5% (w/v) betaine, 150 mM NaCl, 0.02% (w/v) NaN3, 1 mM EDTA, 1 mM PMSF, 15% v/v glycerol, 1 month, 40% loss of activity Neocallimastix sp. MC-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-xylopyranosyl-(1->2)-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O
-
 Neocallimastix sp. MC-2 beta-D-xylopyranosyl-(1->2)-alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + (2E)-4-hydroxycinnamate
-
 ?
additional information p-coumaroyl tetrasaccharides isolated from plant cell walls are hydrolyzed at rates similar to that for PAXX, whereas a dimer of PAXX is hydrolyzed at a rate 20fold lower, yielding 4,4'-dihydroxy-alpha-truxillic acid as an end product. Ethyl and methyl p-coumarates are hydrolyzed at very slow rates, if at all. No substrates: coastal Bermuda grass cell walls, acetylxylan, xylan, avicel, carboxymethyl cellulose, 4-nitrophenyl acetate, 4-nitrophenyl glucopyranoside, 4-nitrophenyl xylopyranoside, 4-nitrophenyl arabinofuranoside Neocallimastix sp. MC-2 ?
-
 ?
O-beta-D-xylopyranosyl-(1->4)-O-[5-O-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl(1->3)]-O-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O
-
 Neocallimastix sp. MC-2 O-beta-D-xylopyranosyl-(1->4)-O-(alpha-L-arabinofuranosyl(1->3))-O-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + (2E)-4-hydroxycinnamate
-
 ?
[5-O-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O i.e. PAXX Neocallimastix sp. MC-2 alpha-L-arabinofuranosyl-(1->3)-O-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + (2E)-4-hydroxycinnamate
-
 ?
[5-O-feruloyl-alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O i.e. FAXX, very low activity Neocallimastix sp. MC-2 ferulic acid + alpha-L-arabinofuranosyl-(1->3)-O-beta-D-xylopyranosyl-(1->4)-D-xylopyranose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 5800, SDS-PAGE Neocallimastix sp. MC-2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Neocallimastix sp. MC-2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 6 h, more than 90% activity Neocallimastix sp. MC-2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Neocallimastix sp. MC-2

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.5
-
 Neocallimastix sp. MC-2

pI Value

Organism Comment pI Value Maximum pI Value
Neocallimastix sp. MC-2 isoelectric focusing
-
 4.7

General Information

General Information Comment Organism
physiological function the purified esterase releases p-coumaroyl groups from finely, but not coarsely, ground plant cell walls, and this activity is enhanced by the addition of xylanase and other cell wall-degrading enzymes Neocallimastix sp. MC-2