Literature summary for 3.1.1.B10 extracted from

Borneman, W.S.; Hartley, R.D.; Himmelsbach, D.S.; Ljungdahl, L.G.
Assay for trans-p-coumaroyl esterase using a specific substrate from plant cell walls (1990), Anal. Biochem., 190, 129-133.

Search for more literature for 3.1.1.B10

Application

Application	Comment	Organism
analysis	assay for catalytic activity using reverse-phase HPLC analysis of 4-coumaric acid released. As little as 100 ng of acid can be determined	Neocallimastix sp. MC-2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0132	-	[5-O-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose	pH 7.5, 30°C	Neocallimastix sp. MC-2

Organism

Organism	UniProt	Comment	Textmining
Neocallimastix sp. MC-2	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[5-O-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O	-	Neocallimastix sp. MC-2	alpha-L-arabinofuranosyl-(1->3)-O-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + (2E)-4-hydroxycinnamate	release of trans-p-coumarate follows Michalis-Menten kinetics	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Neocallimastix sp. MC-2

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Neocallimastix sp. MC-2

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Release 2023.1 (January 2023)