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Literature summary for 3.1.1.96 extracted from
- GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase (2007), Nucleic Acids Res., 35, 930-938.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
D-tyrosyl-tRNATyr | pH 7.8, 27°C | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc ions are essential to the activity of the protein | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34726 | - |
x * 34726, calculated from sequence | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9ZPQ3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartyl-tRNAAsp + H2O | initial rate of hydrolysis is very close to that obtained with D-tyrosyl-tRNATyr | Arabidopsis thaliana | D-aspartate + tRNAAsp | - |
? | |
| D-tyrosyl-tRNATyr + H2O | no activity with L-tyrosyl-tRNATyr and diacetyl-L-Lys-tRNALys | Arabidopsis thaliana | D-tyrosine + tRNATyr | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34726, calculated from sequence | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-aminoacyl-tRNA deacylase | - |
Arabidopsis thaliana |
| GEK1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 300 | - |
D-tyrosyl-tRNATyr | pH 7.8, 27°C | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 272700 | - |
D-tyrosyl-tRNATyr | pH 7.8, 27°C | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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