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Literature summary for 3.1.1.93 extracted from

  • Iwamura, A.; Fukami, T.; Higuchi, R.; Nakajima, M.; Yokoi, T.
    Human alpha/beta hydrolase domain containing 10 (ABHD10) is responsible enzyme for deglucuronidation of mycophenolic acid acyl-glucuronide in liver (2012), J. Biol. Chem., 287, 9240-9249.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) the recombinant enzyme is completely inhibited at 1 mM Homo sapiens
AgNO3 the recombinant enzyme is completely inhibited at 0.1 mM Homo sapiens
bis-4-nitrophenylphosphate the recombinant enzyme shows about 20% residual activity at 1 mM Homo sapiens
CdCl2 the recombinant enzyme shows about 20% residual activity at 1 mM Homo sapiens
CuCl2 the recombinant enzyme shows about 20% residual activity at 1 mM Homo sapiens
diisopropyl fluorophosphate the recombinant enzyme shows about 63% residual activity at 1 mM Homo sapiens
Disulfiram the recombinant enzyme shows about 23% residual activity at 1 mM Homo sapiens
eserine the recombinant enzyme shows about 85% residual activity at 1 mM Homo sapiens
additional information the recombinant enzyme is not inhibited by CoCl2, CaCl2, D-saccharic acid 1,4-lactone, and sodium fluoride Homo sapiens
N-ethylmaleimide the recombinant enzyme shows about 75% residual activity at 1 mM Homo sapiens
phenylmethylsulfonyl fluoride the recombinant enzyme shows about 25% residual activity at 1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1007
-
mycophenolic acid O-acyl-glucuronide recombinant enzyme, in 100 mM Tris-HCl (pH 7.4), at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
microsome
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, precursor protein, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NUJ1
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, CM Sepharose column chromatography, Mono P column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00017
-
unpurified enzyme from cytosol, in 100 mM Tris-HCl (pH 7.4), at 37°C Homo sapiens
0.00954
-
after 55.1fold purification, in 100 mM Tris-HCl (pH 7.4), at 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mycophenolic acid O-acyl-glucuronide + H2O
-
Homo sapiens mycophenolic acid + D-glucuronate
-
?

Subunits

Subunits Comment Organism
? x * 28000, precursor protein, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ABHD10
-
Homo sapiens