Literature summary for 3.1.1.88 extracted from

Zuo, Z.; Gong, T.; Che, Y.; Liu, R.; Xu, P.; Jiang, H.; Qiao, C.; Song, C.; Yang, C.
Engineering Pseudomonas putida KT2440 for simultaneous degradation of organophosphates and pyrethroids and its application in bioremediation of soil (2015), Biodegradation, 26, 223-233 .

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Application

Application	Comment	Organism
agriculture	agricultural soils are often co-contaminated with different types of pesticides. For example, organophosphates and pyrethroids usually are applied together for pest control. So far, microorganisms with both OP- and pyrethroid-degrading activity have not been isolated from natural environments. Alternatively, construction of engineered microbes with broad-spectrum pesticide-degrading activity may be a promising strategy for bioremediation of mixed pesticides-contaminated soil. To construct multifunctional pesticide degrading microorganisms, the co-expression of multiple degrading enzymes in a host strain may be a feasible approach. Both MPH and PytH are coexpressed in a soil bacterium Pseudomonas putida KT2440, resulting in an engineered strain with the capability to simultaneously degrade OPs and pyrethroids. Six pesticides (methyl parathion, fenitrothion, chlorpyrifos, permethrin, fenopropathrin, cypermethrin) can be effectively degraded by the engineered Pseudomonas putida KT2440. Therefore, the engineered Pseudomonas putida KT2440 could potentially be applied for in situ bioremediation of soil co-contaminated with organophosphates and pyrethroids	Sphingobium wenxiniae

Cloned(Commentary)

Cloned (Comment)	Organism
functional expression of mpd and pytH in Pseudomonas putida KT2440 under the control of a constitutive BioBrick promoter J23119	Sphingobium wenxiniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	PytH-His6 fusion protein, SDS-PAGE	Sphingobium wenxiniae

Organism

Organism	UniProt	Comment	Textmining
Sphingobium wenxiniae	C0LA90	-	-
Sphingobium wenxiniae DSM 21828	C0LA90	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cypermethrin + H2O	-	Sphingobium wenxiniae	3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide	-	?
cypermethrin + H2O	-	Sphingobium wenxiniae DSM 21828	3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide	-	?
fenopropathrin + H2O	-	Sphingobium wenxiniae	3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide	-	?
fenopropathrin + H2O	-	Sphingobium wenxiniae DSM 21828	3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide	-	?
permethrin + H2O	-	Sphingobium wenxiniae	3-phenoxybenzyl alcohol + 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate	-	?
permethrin + H2O	-	Sphingobium wenxiniae DSM 21828	3-phenoxybenzyl alcohol + 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate	-	?

Subunits

Subunits	Comment	Organism
?	x * 31000, PytH-His6 fusion protein, SDS-PAGE	Sphingobium wenxiniae

Synonyms

Synonyms	Comment	Organism
pytH	-	Sphingobium wenxiniae

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Release 2023.1 (January 2023)