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Literature summary for 3.1.1.88 extracted from
- Identification and characterization of a novel thermostable pyrethroid-hydrolyzing enzyme isolated through metagenomic approach (2012), Microb. Cell Fact., 11, 33.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0141 | - |
4-nitrophenyl acetate | pH 6.5, 55°C | Escherichia coli |  |
| 0.0316 | - |
4-nitrophenyl butyrate | pH 6.5, 55°C | Escherichia coli | |
| 0.0332 | - |
4-nitrophenyl caprylate | pH 6.5, 55°C | Escherichia coli | |
| 0.0576 | - |
4-nitrophenyl caproate | pH 6.5, 55°C | Escherichia coli | |
| 0.0997 | - |
4-nitrophenyl decanoate | pH 6.5, 55°C | Escherichia coli | |
| 0.13 | - |
4-nitrophenyl laurate | pH 6.5, 55°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30800 | - |
calculated from cDNA | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49.1 | - |
substrate: 4-nitrophenyl laurate, pH 6.5, 55°C | Escherichia coli |
| 69.1 | - |
substrate: 4-nitrophenyl decanoate, pH 6.5, 55°C | Escherichia coli |
| 88.9 | - |
substrate: 4-nitrophenyl caproate, pH 6.5, 55°C | Escherichia coli |
| 149.7 | - |
substrate: 4-nitrophenyl caprylate, pH 6.5, 55°C | Escherichia coli |
| 163.3 | - |
substrate: 4-nitrophenyl butyrate, pH 6.5, 55°C | Escherichia coli |
| 772.9 | - |
substrate: 4-nitrophenyl acetate, pH 6.5, 55°C | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C,10 weeks, 32% loss of activity | Escherichia coli |
| 4°C, 10 weeks, 6% loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Escherichia coli | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Escherichia coli | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caproate + H2O | - |
Escherichia coli | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Escherichia coli | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl decanoate + H2O | - |
Escherichia coli | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Escherichia coli | 4-nitrophenyl + laurate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Sys410 | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme ist stable at temperatures below 50°C | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 49.1 | - |
4-nitrophenyl laurate | pH 6.5, 55°C | Escherichia coli | |
| 69.1 | - |
4-nitrophenyl decanoate | pH 6.5, 55°C | Escherichia coli | |
| 88.9 | - |
4-nitrophenyl caproate | pH 6.5, 55°C | Escherichia coli | |
| 149.7 | - |
4-nitrophenyl caprylate | pH 6.5, 55°C | Escherichia coli | |
| 163.3 | - |
4-nitrophenyl butyrate | pH 6.5, 55°C | Escherichia coli | |
| 289.1 | - |
4-nitrophenyl acetate | pH 6.5, 55°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | - |
Escherichia coli |
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